Anti-SOX-9 Antibody, clone 2B10, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB5535 - recombinant, expressed in HEK 293 cells
Synonym: Transcription factor SOX-9
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) E16.5 mouse embryo (A) and Negative control (B) sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing E16.5 mouse tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) E16.5 mouse embryo (A) and Negative control (B) sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing E16.5 mouse tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) E16.5 mouse embryo (A) and Negative control (B) sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing E16.5 mouse tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) developing mouse nasal cavity (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing mouse nasal cavity tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) developing mouse nasal cavity (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing mouse nasal cavity tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) developing mouse nasal cavity (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing mouse nasal cavity tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) developing mouse gut (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing mouse gut tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) developing mouse gut (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing mouse gut tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) developing mouse gut (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear staining was observed in subsets of tissue types of developing mouse gut tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SW480 cells was performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SW480 (A and B) cell line were performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SW480 (A and B) cell line were performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SW480 (A and B) cell line were performed using a 1:100 dilution of Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from NIH3T3 (A) and HepG2 (B) cells were probed with Cat. No. ZRB5535, Anti-SOX-9, clone 2B10, ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SOX-9 (~65 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 2B10, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 56.14 kDa |
| observed mol wt ~65 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable using 1:100 |
| immunohistochemistry: suitable using 1:100 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | P48436  |
| Application: | Anti-SOX-9, clone 2B10, ZooMAb is a recombinant Rabbit Monoclonal antibody that detects Sox-9 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SOX-9 in HepG2 cell lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SOX-9 in SW480 cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SOX-9 in E16.5 mouse embryo, mouse nasal cavity, and developing mouse gut tissue sections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human SOX-9. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2B10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine SOX-9. It targets an epitope with in 24 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Transcription factor SOX-9 (UniProt P48436; also known SOX-9) is encoded by the SOX9 gene (Gene ID: 6662) in human. SOX family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at about 40 genomic loci and at least 30 SOX genes have been identified. SOX-9 contains one HMG box DNA-binding domain and acts as a key regulator of developmental processes, including male sex determination, normal skeletal development, chondrogenesis, neurogenesis, and neural crest development. SOX-9 is acts downstream of sonic hedgehog signaling and is shown to be essential for the maintenance of multipotent neural stem cells in embryonic and adult central nervous system (CNS). It is not detected in neuroblasts of the adult subependymal zone (SEZ); however, it is expressed by transit amplifying cells and ependymal cells in the same region. SOX-9 is also shown to be essential for the ′switch′ from neurogenesis to gliogenesis in caudal regions of the developing CNS. Up-regulation of SOX-9 is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Scott, CE et al. (2010). Nat. Neurosci. 13(10); 1181-89). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |