Anti-Calretinin Antibody, clone 3K22, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB5054 - recombinant, expressed in HEK 293 cells
Synonym: CR
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB5054, Anti-Calretinin, clone 3K22, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB5054, Anti-Calretinin, clone 3K22, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB5054, Anti-Calretinin, clone 3K22, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in human cerebral cortex tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB5054, Anti-Calretinin, clone 3K22, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB5054, Anti-Calretinin, clone 3K22, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB5054, Anti-Calretinin, clone 3K22, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from brain tissue of Rat (Lane 1), Mouse (Lane 2), and Human (Lane 3) were probed with Cat. No. ZRB5054, Anti-Calretinin, clone 3K22, ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Calretinin (~30 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 3K22, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 31.4 kDa |
| observed mol wt ~30 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat, human |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable using 1:100 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | P47728  |
| Application: | Anti-Calretinin, clone 3K22, ZooMAb, Cat. No. ZRB5054, is a recombinant rabbit monoclonal antibody that detects Calretinin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1000 dilution from a representative lot detected Calretinin in rat and human brain tissue lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calretinin in PC12 cells. Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Calretinin in human cerebral cortex tissue sections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | GST/His-tagged recombinant fragment corresponding to 99 amino acids from the internal region of rat Calretinin. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3K22 is a ZooMAb rabbit recombinant monoclonal antibody that detects Calretinin. It targets an epitope with in the internal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Calretinin (UniProt: P47728; also known as CR) is encoded by the Calb2 gene (Gene ID: 117059) in rat. Calretinin belongs to the calbindin protein family and acts as a calcium-binding protein. It is expressed in both central and peripheral nervous system and is found abundantly in auditory neurons. It is involved in regulating cytosolic calcium ion concentrations and has six calcium-binding domains known as EF-hands. Its expression is shown to be regulated at the level of transcription by nuclear respiratory factor 1 (NRF-1) and E2F2 transcription factors. In rat striatum calretinin neurons form a distinct subset of medium-sized (7- 20 μm), aspiny, GABAergic interneurons. Calretinin can also serve as an important differential diagnostic tool for cystic and solid ameloblastic tumors. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Petryszyn, S., et al. (2017). J. Neural Transm. doi: 10.1007/s00702-017-1687; Kresoja-Rakic, J., et al. (2017). Front. Genet. doi: 10.3389/fgene.2017.00070) |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |