Anti-NeuN Antibody, clone 13E6, ZooMAb^® Rabbit Monoclonal

SIGMA/ZRB377 - recombinant, expressed in HEK 293 cells

Synonym: Fox-1 homolog C; Fox-3; Hexaribonucleotide-binding protein 3; NeuN; Neuronal nuclei; RNA binding protein fox-1 homolog 3

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-ZRB377-25UL	25 µL	$223.00 1/EA	Add To Favorites
45-ZRB377-4X25UL	25 µL	$460.00 1/EA	Add To Favorites

Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse midbrain tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 Dilution of Cat. No. ZRB377, Anti-NeuN, clone 13E6, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear/cytoplasmic staining was observed in neurons of mouse midbrain tissue sections.

Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse cerebral cortex tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 Dilution of Cat. No. ZRB377, Anti-NeuN, clone 13E6, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear/cytoplasmic staining was observed in neurons of mouse brain, hippocampus tissues sections.

Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse brain, hippocampus tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 Dilution of Cat. No. ZRB377, Anti-NeuN, clone 13E6, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear/cytoplasmic staining was observed in neurons of mouse cerebral cortex tissues sections.

Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and human cerebellum (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 Dilution of Cat. No. ZRB377, Anti-NeuN, clone 13E6, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Nuclear/cytoplasmic staining was observed in neurons of human cerebral cortex and human cerebellum tissues sections.

Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of E18 rat cortical cells was performed using a 1:100 dilution of Cat. No. ZRB377, Anti-NeuN, clone 13E6, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus with some cytoplasm.

Immunofluorescence
Enhanced Validation-Recombinant Antibody Technology
Confocal fluorescent analysis of mouse hippocampus FFPE (A) and mouse cerebral cortex frozen (B) sections were performed using of Cat. No. ZRB377, Anti-NeuN, clone 13E6, ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 dye (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus with some cytoplasm.

Western Blotting Enhanced Validation-Recombinant Antibody Technology Mouse brain (Lane 1) and Rat brain (Lane 2) nuclear extracts were probed with Cat. No. ZRB377, Anti-NeuN, clone 13E6, ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NeuN (~46/48 kDa).

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified antibody
antibody product type	primary antibodies
biological source	rabbit (recombinant)
clone	13E6, monoclonal
	recombinant monoclonal
conjugate	unconjugated
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
form	lyophilized
greener alternative category	Aligned
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
isotype	IgG
mol wt	calculated mol wt 40.61 kDa
	observed mol wt ~46/48 kDa
packaging	antibody small pack of 25 μL
product line	ZooMAb^® learn more
recombinant	expressed in HEK 293 cells
shipped in	ambient
species reactivity	rat, mouse, human
storage temp.	2-8°C
technique(s)	immunocytochemistry: suitable using 1:100
	immunofluorescence: suitable using 1:100
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
	western blot: suitable using 1:1,000
UniProt accession no.	Q8BIF2

Application:	Anti-NeuN, clone 13E6, ZooMAb, Cat. No. ZRB377, is a highly specific recombinant Rabbit Monoclonal antibody that detects Neuronal nuclei antigen and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Application:	Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected NeuN in Rat brain nuclear extract. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse hippocampus FFPE and mouse cerebral cortex frozen sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NeuN in E18 rat cortical cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse brain and human brain tissue sections.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
General description:	ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here.
Immunogen:	GST/His-tagged recombinant fragment corresponding to the first 97 amino acids from the N-terminal region of murine Neuronal nuclei antigen (NeuN).
Legal Information:	ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution:	30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity:	Clone 13E6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Neuronal nuclei antigen. It targets an epitope with in the first 97 amino acids from the N-terminal region.
Storage and Stability:	Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description:	RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. The NeuN protein is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord. However, it is not detected in Cajal-Retzius cells in the neocortex, Purkinje cells, and photoreceptor cells. NeuN expression has been reported as early as embryonic day 9.5 (E9.5) and by E12.5 it can be found in the developing ventral horns and is also detected in the developing dorsal horns as well as in the dorsal root ganglion. It is expressed from embryonic stage to adulthood. Its expression is enhanced by retinoic acid treatment. It can serve as a pre-mRNA alternative splicing regulator and regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay. Initial characterization of NeuN was derived from usage of a monoclonal antibody (A60), but later identified as RBFOX3. Subsequent studies have shown that anti-NeuN antibodies can identify most types of neurons in the whole nervous system with some rare exceptions. NeuN knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume. They show increased susceptibility to seizures and reduced anxiety-related behaviors compared with wild type littermates. Antibodies for NeuN predominantly associate with cell nuclei and, to a lesser extent, with the perinuclear cytoplasm. Two isoforms of the NeuN protein (46 and 48 kDa) are present in both locations but differ in their relative concentration in the nucleus and cytoplasm. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lin, YS et al. (2018). PLOS One. 13(2); e0192355; Mao, S., et al. (2016). Front. Neuroanat. 13;10:54).

WGK Germany

WGK 1

Storage Temp.	2-8°C
UNSPSC	12352203

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