Anti-GAPDH Antibody, clone 10B13, ZooMAb^® Rabbit Monoclonal

SIGMA/ZRB374 - recombinant, expressed in HEK 293 cells

Synonym: EC: 1.2.1.12; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-ZRB374-25UL	25 µL	$223.00 1/EA	Add To Favorites
45-ZRB374-4X25UL	25 µL	$460.00 1/EA	Add To Favorites

Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human testis (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB374, Anti-GAPDH, clone 10B13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous staining was observed in seminiferous tubules, while cytoplasmic/nuclear staining was observed in Leydig cells of human testis.

Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal analysis of human testis tissue sections was performed using a 1:100 dilution of Cat. No. ZRB374, Anti-GAPDH, clone 10B13 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.

Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from HeLa cells (Lane 1) and HEK293 cells (Lane 2) were probed with Cat. No. ZRB374, Anti-GAPDH, clone 10B13 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates GAPDH (~36 kDa).

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified antibody
antibody product type	primary antibodies
biological source	rabbit (recombinant)
clone	10B13, monoclonal
	recombinant monoclonal
conjugate	unconjugated
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
form	lyophilized
greener alternative category	Aligned
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
isotype	IgG
mol wt	calculated mol wt 36.05 kDa
	observed mol wt ~36 kDa
packaging	antibody small pack of 25 μL
product line	ZooMAb^® learn more
recombinant	expressed in HEK 293 cells
shipped in	ambient
species reactivity	human
storage temp.	2-8°C
technique(s)	immunofluorescence: suitable using 1:100
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
	western blot: suitable using 1:1,000
UniProt accession no.	P04406

Application:	Anti-GAPDH, clone 10B13 ZooMAb, Cat. No. ZRB374, is a a recombinant Rabbit Monoclonal Antibody that specifically targets Glyceraldehyde-3-phosphate dehydrogenase and ha been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Wetern Blotting.
Application:	Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAPDH in HEK293 cell lysate. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
General description:	ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here.
Immunogen:	GST/His-tagged recombinant fragment corresponding to 190 amino acids from the C-terminal half of human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Legal Information:	ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution:	30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity:	Clone 10B13 is a ZooMab rabbit recombinant monoclonal antibody that specifically detects human Glyceraldehyde-3-phosphate dehydrogenase and targets an epitope with in the C-terminal half.
Storage and Stability:	Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description:	Glyceraldehyde-3-phosphate dehydrogenase (UniProt: P04406; also known as EC: 1.2.1.12, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH) is encoded by the GAPDH (also known as GAPD, CDABP0047, OK/SW-cl.1) gene (Gene ID: 2597) in human. GAPDH is considered as a ′house-keeping′ enzyme that catalyzes the synthesis of 1,3-biphosphoglycerate. It is a homotetrameric enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. Hence, it plays a role in both glycolysis and nuclear functions. It translocates to the nucleus following S-nitrosylation on cysteine 152 that leads to its interaction with SIAH1, which contains a nuclear localization signal. In the nucleus it participates in transcription, RNA transport, DNA replication and apoptosis. Sulfhydration at cysteine 152 is reported to increase its catalytic activity. Its nuclear functions are attributed to its nitrosylase activity, which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and DNA-PK. Oxidative stress is reported to promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process known as nucleocytoplasmic coagulation. These aggregates are observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at methionine 46 plays an essential role in the formation of these insoluble structures. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352203

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