Anti-Connexin-36 Antibody, clone 2D22 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB2421 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from PANC-1 cells (Lane 1), Rat brain tissue (Lane 2), and Human brain tissue (Lane 3) were probed with Cat. No. ZRB2421, Anti-Connexin-36, clone 2D22 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Connexin-36 (~38 kDa).
Western Blotting Enhanced Validation - Recombinant Antibody Technology Recombinant bovine Connexin-36 was probed with Cat. No. ZRB2421, Anti-Connexin-36, clone 2D22 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Connexin-36 (~40 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of non-specific control peptide (Run 1) or Connexin-36 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB2421, Anti-Connexin-36, clone 2D22 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Connexin-36 peptide displayed a KD of 1.1 x 10-6 whereas binding to non-specific control peptide displayed a KD of 5.7 x 10-3.
ELISA Enhanced Validation - Recombinant Antibody Technology 50 ng of Connexin-36 peptide (Blue line) and a non-specific control peptide (Orange line) were analyzed by ELISA using various dilutions (starting at 3 g/mL) of Anti-Connexin-36, clone 2D22 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2D22, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 36.09 kDa |
| observed mol wt ~38 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_065711.1  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | bovine, human, rat |
| species reactivity (predicted by homology) | feline, porcine, monkey, mouse, canine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9UKL4 |
| Application: | Quality Control Testing Evaluated by Western Blotting in PANC-1 cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Connexin-36 in PANC-1 cell lysate. Tested applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Connexin-36 in lysates from Rat brain tissue, Human brain tissue and recombinant Bovine Connexin-36. Enzyme Immunoassay Analysis (ELISA): A representative lot of this antibody detected Connexin-36 peptide in ELISA application. Affinity Binding Assay: A representative lot of this antibody bound Connexin-36 peptide with a KD of 1.1 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal, cytoplasmic domain of human Connexin-36. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2D22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Connexin 36. It targets an epitope within 17 amino acids from the C-terminal, cytoplasmic domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Gap junction delta-2 protein (UniProt: Q9UKL4; also known as Connexin-36, Cx36, Gap junction alpha-9 protein) is encoded by the GJD2 (also known as GJA9) gene (Gene ID: 57369) in human. Cx36 is a multi-pass membrane protein with 3 cytoplasmic domains, 4 transmembrane domains, and 2 extracellular domains. It is mainly expressed in neurons where it forms electrical synapses and in pancreatic β cells where it plays an important role in insulin secretion and glycemic control. It is regulated by intracellular pH and cytosolic magnesium ions, which can vary significantly under various physiological and pathological conditions. Its levels are upregulated under conditions of low intracellular pH and Mg2+. It exhibits a very low unitary conductance and low sensitivity to transjunctional voltage. Cx36 knockout (Cx36 -/-) mice exhibit impaired visual transmission and elevated auditory brainstem response threshold. Deletion of Cx36 in mice is shown to cause the loss of functional synapses between GABAergic interneurons in hippocampal slices. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rimkute, L., et al. (2018). Front. Physiol. 9; 362). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |