Anti-IL-17A Antibody, clone 3K22 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB2409 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse spleen (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in both red & white pulp of mouse spleen sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse spleen (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in both red & white pulp of mouse spleen sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse spleen (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in both red & white pulp of mouse spleen sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in lamina propria of human colon tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in lamina propria of human colon tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of EL-4 cells was performed using a 1:100 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of EL-4 cells was performed using a 1:100 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of EL-4 cells was performed using a 1:100 dilution of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 μg/mL of non-specific GST protein (Run 1) or recombinant mouse IL-17A protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal. The binding of this antibody to IL-17A protein displayed a KD of 2.5 x 10-8 whereas binding to non-specific GST protein displayed a KD of 1.1 x 10-4.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million EL4 cells was performed using 0.1 μg of Cat. No. ZRB2409, Anti-IL-17A, clone 3K22 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 3K22, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 17.49 kDa |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_034682.1  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| UniProt accession no. | Q62386 |
| Application: | Quality Control Testing Evaluated by Immunohistochemistry (Paraffin) in Human spleen tissue sections. Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:1,000 dilution of this antibody detected IL-17A in mouse spleen tissue sections. Tested applications Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IL-17A in human colon tissue sections. Affinity Binding Assay: A representative lot of this antibody bound IL-17A with a KD of 2.5 x 10-8 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IL-17A in EL-4 cell line. Flow Cytometry Analysis: 0.1 μg from a representative lot detected IL-17A in one million EL4 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | Full-length recombinant mouse IL-17A expressed in CHO cells. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3K22 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects IL-17A. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Interleukin-17A (UniProt: Q62386; also known as IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8) is encoded by the Il17A (also known as Ctla8, Il17) gene (Gene ID: 16171) in murine species. IL-17A is a homodimeric, proinflammatory cytokine that is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off to produce the mature protein. It is expressed by Th17 cell lineage, and its expression is observed in innate lymphoid cells, T cell subsets, and invariant natural killer (iNKT) cells. It is induced upon differentiation of CD4+ T cells toward Th17 effector cells upon antigen receptor binding in the presence of IL6 and TGF-β1. Its levels are also up-regulated by proinflammatory cytokines in response to microbes in various immune cells. IL-17A actions are mediated via interaction with IL-17RA and IL-17RC heterodimeric receptor complex, triggering homotypic interaction of these receptors with TRAF3 Interacting Protein 2 (TRAF3IP2) adapter. This leads to downstream TRAF6-mediated activation of NF-kB and MAP kinase pathways resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases. IL-17A plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It plays important roles in multiple biological processes, including the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF- kB and co-stimulation of T cell proliferation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liang, SC., et al. (2007). J. Immunol. 179(11); 7791-7799; Park, H., et al. (2005). Nat. Immunol. 6(11); 1133-1141). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |