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Anti-Tyrosine Hydroxylase Antibody, clone 3M12, ZooMAb® Rabbit Monoclonal

SIGMA/ZRB2381 - recombinant, expressed in HEK 293 cells

Synonym: EC: 1.14.62.2; TH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-ZRB2381-25UL 25 µL
$223.00
1/EA
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45-ZRB2381-4X25UL 25 µL
$460.00
1/EA
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Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and mouse brain striatum (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in nerve fibers of mouse brain, striatum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and mouse brain striatum (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in nerve fibers of mouse brain, striatum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and mouse brain striatum (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in nerve fibers of mouse brain, striatum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and mouse brain isocortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in nerve fibers of mouse brain, isocortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and mouse brain isocortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in nerve fibers of mouse brain, isocortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and mouse brain isocortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in nerve fibers of mouse brain, isocortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous /nuclear staining was observed in subsets of neurons and glial cells, as well as neuropil of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous /nuclear staining was observed in subsets of neurons and glial cells, as well as neuropil of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous /nuclear staining was observed in subsets of neurons and glial cells, as well as neuropil of human cerebral cortex tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of mouse brain, isocortex (A), mouse brain striatum (B), and rat cerebral cortex tissue sections were performed using a 1:10 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of mouse brain, isocortex (A), mouse brain striatum (B), and rat cerebral cortex tissue sections were performed using a 1:10 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of mouse brain, isocortex (A), mouse brain striatum (B), and rat cerebral cortex tissue sections were performed using a 1:10 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of mouse brain, isocortex (A), mouse brain striatum (B), and rat cerebral cortex tissue sections were performed using a 1:10 dilution of Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology PC12 cell lysate was probed with Cat. No. ZRB2381, Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Tyrosine Hydroxylase (~58 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention.  This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency.  This product was re-engineered to utilize less energy in the manufacturing process.

 

antibody form purified antibody
antibody product type primary antibodies
biological source rabbit (recombinant)
clone 3M12, monoclonal
  recombinant monoclonal
conjugate unconjugated
enhanced validation recombinant expression
Learn more about Antibody Enhanced Validation 
form lyophilized
greener alternative category Aligned 
greener alternative product characteristics Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry .
isotype IgG
mol wt calculated mol wt 55.97 kDa
  observed mol wt ~58 kDa
packaging antibody small pack of 25 μL
product line ZooMAb® learn more 
recombinant expressed in HEK 293 cells
shipped in ambient
species reactivity rat, mouse
species reactivity (predicted by homology) human, bovine
storage temp. 2-8°C
technique(s) immunocytochemistry: suitable using 1:100
  immunofluorescence: 1:10-1:100
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000
  western blot: suitable using 1:1,000
UniProt accession no. P04177 
Application: Anti-Tyrosine Hydroxylase, clone 3M12 ZooMAb, Cat. No. ZRB2381, is a recombinant Rabbit Monoclonal Antibody that specifically targets Tyrosine 3-hydroxylase and has been tested for use in Immunocytochemistry, Immunfluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Application: Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected Tyrosine Hydroxylase in mouse brain and rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tyrosine Hydroxylase in PC12 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Tyrosine Hydroxylase in mouse brain tissue sections.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here  for more information.
General description: ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here. 
Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of rat Tyrosine 3-hydroxylase.
Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution: 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity: Clone 3M12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Tyrosine Hydroxylase. It targets an epitope with in 15 amino acids from the N-terminal region.
Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description: Tyrosine 3-monooxygenase (UniProt: P04177; also known as EC: 1.14.62.2, Tyrosine 3-hydroxylase, TH) is encoded by the TH gene (Gene ID: 25085) in rat. Tyrosine hydroxylase acts as a rate-limiting enzyme of catecholamine biosynthesis. It hydroxylates tyrosine to L-DOPA and then L- DOPA is converted to dopamine by aromatic amino acid decarboxylase. Tyrosine hydroxylase requires tetrahydrobiopterin and molecular oxygen to convert tyrosine to DOPA. Its activity is regulated by phosphorylation at four different serine residues by multiple protein kinases. The N-terminal region of tyrosine hydroxylase plays a critical role in controlling its catalytic activity. Its catalytic domain is located in the C-terminal region and binds the substrates and the cofactor. Its activity is inhibited by a feedback mechanism by catecholamines. Dopamine is shown to bind to tyrosine hydroxylase competitively with tetrahydrobiopterin and interacts with the regulatory domain. Tyrosine hydroxylase activity can be modified in the presence of nitric oxide that results in nitration of tyrosine residues. Tyrosine hydroxylase activity and dopamine levels are shown to be significantly lower in the brain of Parkinson s disease subjects. Tyrosine hydroxylase deficiency leads to impaired synthesis of dopamine as well as epinephrine and norepinephrine. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Daubner SC et al., (2011). Arch. Biochem. Biophys. 508(1): 1-12).
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352203

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