Anti-VGluT1 Antibody, clone 4O15, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB2374 - recombinant, expressed in HEK 293 cells
Synonym: Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7; Vesicular glutamate transporter 1
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and rat midbrain (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of rat midbrain tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and rat midbrain (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of rat midbrain tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and rat midbrain (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of rat midbrain tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and rat cerebellum (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and rat cerebellum (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and rat cerebellum (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of rat cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of human cerebral cortex tissue sections
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of human cerebral cortex tissue sections
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) negative control (A) and human cerebral cortex (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Membranous staining was observed in areas of human cerebral cortex tissue sections
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat cerebellum (A), rat hindbrain (B), and human cerebral cortex (C) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat cerebellum (A), rat hindbrain (B), and human cerebral cortex (C) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat cerebellum (A), rat hindbrain (B), and human cerebral cortex (C) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Immunofluorescence Enhanced Validation-Recombinant Antibody Technology Confocal fluorescent analysis of rat cerebellum (A), rat hindbrain (B), and human cerebral cortex (C) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Rat brain tissue lysate was probed with Cat. No. ZRB2374, Anti-VGluT1, clone 4O15 ZooMAb® Rabbit Monoclonal (1:4,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates VGluT1 (~53 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 4O15, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 61.66 kDa |
| observed mol wt ~53 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse, human |
| storage temp. | 2-8°C |
| technique(s) | immunofluorescence: suitable using 1:100 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | Q62634  |
| Application: | Anti-VGluT1, clone 4O15 ZooMAb, Cat. No. ZRB2374, is a recombinant Rabbit Monoclonal Antibody that specifically targets Vesicular Glutamate Transporter 1 (aka Slc17a7 or VGlut1) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected VGluT1 in rat brain and human brain tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected VGluT1 in rat brain and human brain tissue sections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of rat Vesicular glutamate transporter 1 (VGlut1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4O15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Vesicular glutamate transporter 1. It targets an epitope with in 19 amino acids from the C-terminal cytoplasmic domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Vesicular glutamate transporter 1 (UniProt: also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID: 116638) in rat. VGluT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neuronal cells. Glutamate transporters mediate glutamate uptake into synaptic vesicles and are driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGluT1 have been reported that are produced by alternative splicing. VGluT1 is expressed in the cerebellum, cerebral cortex and hippocampus. Expression of isoform VGluT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGluT1 and VGluT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGluT2 expression predominates. VGluT1 expression becomes essential for survival at the time of the developmental switch from VGluT2 to VGLUT1. The VGluT1 knockout mice die at the age when a strong upregulation of VGluT1 through newly emerging VGluT1 synapses as well as a re+D4placement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18): 7158-7163). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |