Anti-USP7 Antibody, clone 1E19 ZooMAb^® Rabbit Monoclonal

SIGMA/ZRB1794 - recombinant, expressed in HEK 293 cells

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-ZRB1794-25UL		No Price
45-ZRB1794-4X25UL		No Price

Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human colon (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1794, Anti-USP7, clone 1E19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in glandular cells & lamina propria of human colon tissue sections.

Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cells was performed using a 1:300 dilution of Cat. No. ZRB1794, Anti-USP7, clone 1E19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.

Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from K562 (Lane 1), U2OS (Lane 2), Jurkat (Lane 3), NIH3T3 (Lane 4), and Cos7 (Lane 5) cells were probed with Cat. No. ZRB1794, Anti-USP7, clone 1E19 ZooMAb® Rabbit Monoclonal (1:3,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates USP7 (~130 kDa).

Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or USP7 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1794, Anti-USP7, clone 1E19 ZooMAb® Rabbit Monoclonal. The binding of this antibody to USP7 peptide displayed a KD of 1.8 x 10-7 whereas binding to non-specific control peptide displayed a KD of 1.0 x 10-2.

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.

Properties
Descriptions
Basic Data

antibody form	purified antibody
antibody product type	primary antibodies
biological source	rabbit
clone	1E19, recombinant monoclonal
conjugate	unconjugated
description	recombinant, expressed in HEK 293 cells
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
epitope sequence	N-terminal
form	lyophilized
greener alternative category	Aligned
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
isotype	IgG
mol wt	calculated mol wt 128.3 kDa
	observed mol wt ~130 kDa
packaging	antibody small pack of 25 μL
product line	ZooMAb^® learn more
Protein ID accession no.	NP_003461
purified by	using Protein A
Quality Level	200
recombinant	expressed in HEK 293 cells
shipped in	ambient
species reactivity	mouse, human, monkey
species reactivity (predicted by homology)	rat, monkey, porcine, feline, bovine
storage temp.	2-8°C
technique(s)	affinity binding assay: suitable
	immunocytochemistry: suitable
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
	western blot: suitable
UniProt accession no.	Q93009

Application:	Quality Control Testing Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: A 1:3,000 dilution of this antibody detected USP7 in K562 cell lysate. Tested applications Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected USP7 in U2OS, Jurkat, NIH3T3, and Cos7 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected USP7 in human colon tissue sections. Immunocytochemistry Analysis: A 1:300 dilution from a representative lot detected USP7 in Jurkat cells. Affinity Binding Assay: A representative lot of this antibody bound USP7 with a KD of 1.8 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
General description:	ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.
Immunogen:	KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Ubiquitin carboxyl-terminal hydrolase 7 (USP7).
Legal Information:	ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution:	300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity:	Clone 1E19 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects USP7. It targets an epitope within 15 amino acids from the N-terminal region.
Storage and Stability:	Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description:	Ubiquitin carboxyl-terminal hydrolase 7 (UniProt: Q93009; also known as EC:3.4.19.12, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7, USP7) is encoded by the USP7 (also known as HAUSP) gene (Gene ID: 7874) in human. USP7 is a widely expressed deubiquitinating enzyme that erases ubiquitin and protects substrate protein from degradation. It displays a preference towards lysine 48-linked ubiquitin chains. It contains an N-terminal poly-glutamine stretch (poly Q), the tumor necrosis factor receptor- associated factors (TRAF) domain (aa 62-205), the catalytic domain (aa 208-560), and the C-terminal tandem ubiquitin-like (Ubl) domain (aa 560-1102). Full activity of USP7 requires the C-terminal Ubl domain that fold back onto the catalytic domain, allowing the remodeling of the active site to a catalytically competent state by the C-terminal peptide. The catalytic domain has a three-domain architecture, including Fingers, Palm, and Thumb domains. 19 residues in the C-terminal region (aa 1084 1102), which are conserved across species are reported to stabilize the ubiquitin binding conformation of the catalytic domain. Individual point mutations at isoleucine residues 1100 or 1098 are able to abolish its deubiquitinase activity. USP7 is known to deubiquitinate multiple target proteins, including FOXO4, p53, DNMT1, PTEN, and DAXX. By preventing the degradation of p53/TP53, it enhances p53/TP53-dependent transcription regulation, cell growth repression, and apoptosis. Overexpression of USP7 has been reported in prostate cancer. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, Z., et al. (2019). Front. Pharmacol. 10; 427; Li, M., et al. (2002). Nature. 416(6881); 648-653).

Storage Temp.	2-8°C
UNSPSC	12352203

To Order From a Quote