Anti-Calbindin D-28K clone 1A11 Antibody, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1778 - recombinant, expressed in HEK 293 cells
Synonym: Calbindin; D-28K; D28; PCD-29; Spot 35 protein; Vitamin D-dependent calcium-binding protein avian-type
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse kidney tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB1778, Anti-Calbindin D-28K, clone 1A11, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in subsets of tubules of mouse kidney.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse kidney tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB1778, Anti-Calbindin D-28K, clone 1A11, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in subsets of tubules of mouse kidney.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse kidney tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:400 dilution of Cat. No. ZRB1778, Anti-Calbindin D-28K, clone 1A11, ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit and DAB. Cytoplasmic/membranous/nuclear staining was observed in subsets of tubules of mouse kidney.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB1778, Anti-Calbindin D-28K, clone 1A11, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB1778, Anti-Calbindin D-28K, clone 1A11, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of PC12 cells was performed using a 1:100 dilution of Cat. No. ZRB1778, Anti-Calbindin D-28K, clone 1A11, ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from brain tissue of Rat (Lane 1) and Mouse (Lane 2) were probed with Cat. No. ZRB1778, Anti-Calbindin D-28K, clone 1A11, ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Calbindin D-28K (~28 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1A11, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 29.99 kDa |
| observed mol wt ~28 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human, rat |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable using 1:100 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400 | |
| western blot: suitable using 1:1,000 | |
| UniProt accession no. | P12658  |
| Application: | Anti-Calbindin D-28K, clone 1A11, ZooMAb, Cat. No. ZRB1778, is a recombinant Rabbit Monoclonal Antibody that detects Calbindin D-28K and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Calbindin D-28K in mouse brain tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Calbindin D-28K in mouse kidney tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calbindin D-28K in PC12 cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | GST/His-tagged recombinant fragment corresponding to 107 amino acids from the C-terminal half of murine Calbindin. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1A11 is a ZooMAb rabbit recombinant monoclonal antibody that detects Calbindin. It targets an epitope with in the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Calbindin (UniProt: P12658; also known as Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type) is encoded by the calb1 gene (gene ID: 12307) in murine species. Calbindin D-28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. Calbindin D-28K has four functional calcium-binding sites (aa 24-35; 111-122; 155-166; and 199-210). It has moderate cytoplasmic mobility and comprises six EF-hands, of which one or two are non-functioning in metal binding. EF-2 is viewed as non-metal binding and EF-6 may have a very low Ca2+ affinity. Four EF hands function in calcium binding with rapid to intermediate kinetics and affinity. The biological roles of Calbindin D-28K include calcium regulation and signaling, including calcium transport and uptake, calcification of bone and teeth, and calcium related signaling in neurons and transiently in embryological development. It also protects neurons from apoptotic cell death. Decline in Calbindin D-28K levels in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D-28K immunoreactivity is shown to be specifically absent from neuronal populations lost early in ALS, while substantial loss of neurons, which do contain this protein occur in patients with Huntington′s disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Schmidt, H (2012). Front. Mol. Neurosci. 5; 25). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |