Anti-Syk Antibody, clone 2G22 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1776 - recombinant, expressed in HEK 293 cells
Synonym: Tyrosine-protein kinase SYK;EC:2.7.10.2;Spleen tyrosine kinase;p72-Syk
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1776, Anti-Syk, clone 2G22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in both germinal center & non-germinal centers of Human Tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1776, Anti-Syk, clone 2G22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in both germinal center & non-germinal centers of Human Tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1776, Anti-Syk, clone 2G22 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in both germinal center & non-germinal centers of Human Tonsil tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from Ramos cells (Lane 1), A431cells (Lane 2), Raw264.7 cells (Lane 3), and Mouse Spleen tissue (Lane 4) lysates were probed with Cat. No. ZRB1776, Anti-Syk, clone 2G22 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Syk (~70 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Syk peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1776, Anti-Syk, clone 2G22 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Syk peptide displayed a KD of 6.6 x 10-8, whereas binding to non-specific control peptide displayed a KD of 2.1 x 10-5.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million Ramos cells was performed using 1 μg of Cat. No. ZRB1776, Anti-Syk, clone 2G22 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2G22, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 72.07 kDa |
| observed mol wt ~70 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_001167638  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse, rat |
| species reactivity (predicted by homology) | canine, feline, porcine, bovine, monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P43405 |
| Application: | Quality Control Testing Evaluated by Western Blotting in Ramos cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Syk in Ramos cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Syk in lysates from A431 and Raw264.7 cells and Mouse Spleen tissue. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Syk in human tonsil tissue sections. Flow Cytometry Analysis: 1 μg from a representative lot detected Syk in one million Ramos cells. Affinity Binding Assay: A representative lot of this antibody bound Syk with a KD of 6.6 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Tyrosine-protein kinase SYK (Syk). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2G22 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Syk. It targets an epitope within 16 amino acids from the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Tyrosine-protein kinase SYK (UniProt: P43405; also known as EC:2.7.10.2, Spleen tyrosine kinase, p72-Syk) is encoded by the SYK gene (Gene ID: 6850) in human. Syk is a non-receptor tyrosine kinase that is highly expressed in hematopoietic cells. It plays a crucial role not only in adaptive immune receptor signaling, but also in cellular adhesion, innate immune recognition, osteoclast maturation, platelet activation and vascular development. Syk contains two SH2 domains (aa 15-107 and 168-259) that mediate its interaction with the phosphorylated ITAM domains of transmembrane proteins. Its protein kinase domain is localized in amino acids 371-631. Intramolecular binding of the interdomains A and B (aa 108-160 and 260-370) to parts of its catalytic domain keeps the catalytic center in an inactive conformation. The phosphorylation of the interdomains or the binding of the SH2 domains with dually phosphorylated ITAM domains on transmembrane proteins disrupts these intramolecular interactions and allows the kinase domain to adopt an active conformation. Initial ITAM binding triggers rapid activation of Syk, whereas subsequent phosphorylation leads to prolonged activation. The phosphorylation of Syk and of the ITAM domains is essentially mediated by SRC subfamily kinases, like LYN, upon transmembrane receptors engagement. Syk can phosphorylate ITAMs and autophosphorylate its own interdomain region tyrosines, providing a positive feedback loop following initial ITAM-mediated Syk activation. Syk is also reported to mediate signaling of receptors that do not contain an ITAM, including integrins and C-type lectins, via interaction with ITAM-containing adaptors, such as DAP12 (TYROBP) and the FcR -chain (FcR ). This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mocsai, A., et al. (2010). Nat. Rev. Immunol. 10(6); 387-402). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |