Anti-SUZ12 Antibody, clone 2E2 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1773 - recombinant, expressed in HEK 293 cells
Synonym: Polycomb protein SUZ12;Chromatin precipitated E2F target 9 protein;ChET 9 protein:Joined to JAZF1 protein;Suppressor of zeste 12 protein homolog
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Testis (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1773, Anti-SUZ12, clone 2E2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in seminiferous tubules of Human Testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Testis (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1773, Anti-SUZ12, clone 2E2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in seminiferous tubules of Human Testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Testis (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1773, Anti-SUZ12, clone 2E2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in seminiferous tubules of Human Testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Testis (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1773, Anti-SUZ12, clone 2E2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in seminiferous tubules of Human Testis tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Testis (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1773, Anti-SUZ12, clone 2E2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in seminiferous tubules of Human Testis tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from NIH3T3 cells (Lane 1), Jurkat cells (Lane 2), and C6 cells (Lane 3) were probed with Cat. No. ZRB1773, Anti-SUZ12, clone 2E2 ZooMAb® Rabbit Monoclonal (1:1,000 dilution for NIH3T3 and L6 cells; and 1:10,000 dilution for Jurkat cells). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SUZ12 (~84 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or SUZ12 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1773, Anti-SUZ12, clone 2E2 ZooMAb® Rabbit Monoclonal. The binding of this antibody to SUZ12 peptide displayed a KD of 4.0 x 10-7, whereas binding to non-specific control peptide displayed a KD of 1.9 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2E2, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 83.06 kDa |
| observed mol wt ~84 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_056170  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse, rat |
| species reactivity (predicted by homology) | bovine, canine, monkey, feline |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q15022 |
| Application: | Quality Control Testing Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SUZ12 in Jurkat cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SUZ12 in NIH3T3 and C6 cell lysates. Affinity Binding Assay: A representative lot of this antibody bound SUZ12 with a KD of 4.0 x 10-7 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SUZ12 in human testis tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 20 amino acids from N-terminal half of human Polycomb protein SUZ12. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2E2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Polycomb protein SUZ12. It targets an epitope within 20 amino acids from the N-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Polycomb protein SUZ12 (UniProt: Q15022; also known as Chromatin precipitated E2F target 9 protein, ChET 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, SUZ12) is encoded by the SUZ12 (also known as CHET9, JJAZ1, KIAA0160) gene (Gene ID: 23512) in human. SUZ12 is a component of the PRC2 complex, which consists of the core subunits EED, EZH1 or EZH2, SUZ12, and RBBP4, and various combinations of accessory subunits including AEBP2, JARID2, PHF19, MTF2 and EPOP. This complex methylates lysine 9 (H3K9me) and lysine 27 (H3K27me) of histone H3 leading to transcriptional repression of the affected target genes. This complex also serves as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. SUZ12 is shown to be essential for embryonic stem cell (ES) differentiation. It is required for establishing a specific expression program for ES differentiation. Complete loss of SUZ12 results in global loss of H3K27me3 and destabilization of EZH2. SUZ12 -/- mouse embryos die around seven days postcoitus. In quiescent cells, SUZ12 is expressed at low levels and its expression increases at the G1/S phase transition. Overexpression of SUZ12 is reported in several cases of breast and colon cancers. A chromosomal aberration involving SUZ12 has also been linked to endometrial stromal tumors. These tumors are often associated with t(7;17)(p15;q21), resulting in a JAZF1-SUZ12 (JJAZ1) oncogene, which consists of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pasini, D., et al. (2007). Mol. Cell. Biol.27(10); 3769-3779; Lee, SCW., et al. (2005). Blood. 126(2); 167-175; Pasini, D., et al. (2004). EMBO J. 23(20); 4061-4071). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |