Anti-NOVA2 Antibody, clone 2J16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1742 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear/cytoplasmic staining was observed in neurons of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear/cytoplasmic staining was observed in neurons of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear/cytoplasmic staining was observed in neurons of human cerebral cortex tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Neuro-2a cells was performed using a 1:100 dilution of Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Neuro-2a cells was performed using a 1:100 dilution of Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Neuro-2a cells was performed using a 1:100 dilution of Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa FluorZooMAb® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Recombinant Human NOVA2 protein was probed with Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Protein was visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NOVA2 (~52 kDa).
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from E16 Rat brain (Lane 1) and E16 Mouse brain E16 (Lane 2) tissues were probed with Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NOVA2 (~65 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or NOVA2 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1742, Anti-NOVA2, clone 2J16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to NOVA2 peptide displayed a KD of 2.4 x 10-6 whereas binding to non-specific control peptide displayed a KD of 3.7 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2J16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 49.1 kDa |
| observed mol wt ~52 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_002507  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse, human |
| species reactivity (predicted by homology) | porcine, feline, bovine, canine, monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9UNW9 |
| Application: | Quality Control Testing Evaluated by Western Blotting with recombinant Human NOVA2. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant Human NOVA2 protein. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected NOVA2 in lysates from E16 Rat brain, E16 Mouse brain E16, and Human fetal brain tissue.. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NOVA2 in Neuro-2a cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NOVA2 in human cerebral cortex tissue sections. Affinity Binding Assay: A representative lot of this antibody bound NOVA2 with a KD of 2.4 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human RNA-binding protein NOVA2. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2J16 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects RNA-binding protein NOVA2. It targets an epitope within 20 amino acids from the N-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | RNA-binding protein Nova-2 (UniProt: Q9UNW9; also known as Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2) is encoded by the NOVA2 (also known as ANOVA, NOVA3) gene (Gene ID: 4858) in human. The neuron specific RNA-binding proteins NOVA1 and NOVA2 are highly homologous alternative splicing regulators. Both NOVA1 and NOVA2 have three K homology (KH) domains. They are described as autoantigens in the human autoimmune neurological disease known as paraneoplastic opsoclonus-myoclonus ataxia (POMA) that is characterized by loss of motor control in limbs and eyes. NOVA proteins regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. NOVA2 binds RNA with high affinity and with sequence specificity that differs from NOVA1. Its expression is restricted o astrocytes. In close association with NOVA 1, it performs unique biological functions in different brain areas and cell types. NOVA2 binding to an exonic 5′-YCAY-3′ cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5′-YCAY-3′ cluster enhances spliceosome assembly and exon inclusion. It is reported to regulate alternative splicing events of several axon guidance related genes during cortical development. It controls RNA splicing in both inhibitory and excitatory neurons. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Saito, Y., et al. (2016). eLife. 5; e14371; Yang, YYL., et al. (1998). Proc. Natl. Acad. Sci. USA. 95(22); 13254-13259). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |