Anti-ITIH1 Antibody, clone 1K16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1737 - recombinant, expressed in HEK 293 cells
Synonym: Inter-alpha-trypsin inhibitor heavy chain H1;ITI heavy chain H1;ITI-
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Placenta (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chorionic villi (A) and decidua (B) of Human Placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Placenta (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chorionic villi (A) and decidua (B) of Human Placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Placenta (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chorionic villi (A) and decidua (B) of Human Placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Placenta (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chorionic villi (A) and decidua (B) of Human Placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Placenta (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in chorionic villi (A) and decidua (B) of Human Placenta tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Human Placenta tissue sections was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix (ECM).
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Human Placenta tissue sections was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix (ECM).
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Human Placenta tissue sections was performed using a 1:1,000 dilution of Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the extracellular matrix (ECM).
Western Blotting Enhanced Validation - Recombinant Antibody Technology Human plasma (Lane 1) and human serum (Lane 2) were probed with Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ITIH1 (~75, 110, and 250 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or ITIH1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1737, Anti-ITIH1, clone 1K16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to ITIH1-SHAP peptide displayed a KD of 4.8 x 10-7, whereas binding to non-specific control peptide displayed a KD of 3.8 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1K16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 101.39, 86.19, and 69.5 kDa |
| observed mol wt ~250, 110, and 75 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_002206  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P19827 |
| Application: | Quality Control Testing Evaluated by Western Blotting in Human serum. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ITIH1 in Human serum. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ITIH1 in Human plasma. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected ITIH1 in human placenta tissue sections. Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected ITIH1 in human placenta tissue sections. Affinity Binding Assay: A representative lot of this antibody bound ITIH1 with a KD of 4.8 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 20 amino acids the C-terminal region of human mature ITIH1/SHAP (without signal peptide and propeptides). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1K16 is a ZooMAb® Rabbit recombinant monoclonal antibody that detects Inter- a-trypsin inhibitor heavy chain H1 (ITIH1/SHAP). It targets an epitope within 20 amino acids from the C-terminal region of mature ITIH1/SHAP. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Inter-alpha-trypsin inhibitor heavy chain H1 (UniProt: P19827; also known as ITI heavy chain H1, ITI-HC1, Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Serum-derived hyaluronan-associated protein, SHAP) is encoded by the ITIH1 (also known as IGHEP1) gene (Gene ID: 3697) in human. The inter- a-trypsin inhibitor (ITI) family is a group of proteins that are composed of variable associations of light (ITIL) and five heavy chains (ITIH1, -H2, -H3, -H4, -H5), encoded by different genes. They contribute to extracellular matrix stability by covalent linkage to hyaluronan. Heavy chains represent the main structural unit of the ITI complex. The heavy chains are linked to light chain via a single chondroitin sulfate chain, which makes ITI a both structurally and functionally unique proteoglycan with a plasma protease inhibitory activity that resides solely in the light chain. ITIH1 is a glycoprotein that is synthesized in the liver and released into circulation. It is synthesized with a signal peptide (aa 1-27) and two pro-peptides (aa 28-34 and 673-911) that are subsequently cleaved off to generate the mature form (aa 35-672). ITIH1 serves as a carrier of hyaluronan in serum or acts as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis, and degradation of hyaluronan. ITIH1 is suggested to have an antitumor role. It is shown to increase cell attachment and reduce the number of metastases. Down-regulation of ITIH genes have been reported in multiple solid tumors, including breast, colon, and lung cancer. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hamm, A., et al. (2008). BMC Cancer. 8; Article 25; Paris, S., et al. (2002). Int J Cancer. 97(5); 615-620). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |