Anti-PCP4 Antibody, clone 1C4-H2 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1732 - recombinant, expressed in HEK 293 cells
Synonym: Calmodulin regulator protein PCP4;Brain-specific polypeptide PEP-19;Purkinje cell protein 4
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining in both granular & molecular layers, as well as Purkinje cells of Human Cerebellum sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining in both granular & molecular layers, as well as Purkinje cells of Human Cerebellum sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining in both granular & molecular layers, as well as Purkinje cells of Human Cerebellum sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:1,000 dilution of Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:1,000 dilution of Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:1,000 dilution of Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Mouse Brain tissue lysate was probed with Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PCP4 (~12 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or PCP4 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1732, Anti-PCP4, clone 1C4-H2 ZooMAb® Rabbit Monoclonal. The binding of this antibody to PCP4 peptide displayed a KD of 1.8 x 10-7, whereas binding to non-specific control peptide displayed a KD of 3.0 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1C4-H2, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 6.79 kDa |
| observed mol wt ~12 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_006189  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | rat, feline, canine, bovine, horse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P48539 |
| Application: | Quality Control Testing Evaluated by Western Blotting in Mouse Brain tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PCP4 in Mouse Brain tissue lysate. Tested applications Affinity Binding Assay: A representative lot of this antibody bound PCP4 with a KD of 1.8 x 10-7 in an affinity binding assay. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PCP4 in SH-SY5Y cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PCP4 in human cerebellum tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal, IQ domain of human Calmodulin regulator protein PCP4. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1C4-H2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Calmodulin regulator protein PCP4. It targets an epitope within 22 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Calmodulin regulator protein PCP4 (UniProt: P48539; also known as Brain-specific polypeptide PEP-19, Purkinje cell protein 4) is encoded by the PCP4 (also known as PEP19) gene (Gene ID: 5121) in human. Calmodulin (CaM) plays a key role in calcium signaling events and its regulation is an important sically dicomponent of cellular homeostasis. PCP4 is a small IQ motif-containing (aa 39-62), intrinsically disordered protein that functions as a modulator of calcium-binding by CaM. Its acidic region (aa 28-40) is reported to be essential for both modulating calcium-binding to CaM and sensitizing cells to ATP-induced calcium-release. It binds to both calcium-free and calcium-bound forms of CaM, however, its affinity for calcium-bound form is about 50-fold greater. PCP4 is also shown to regulate arrhythmogenicity through a cell-autonomous mechanism within the Purkinje fiber network. It regulates cardiac rhythmicity through modulation of autonomic tone. PEP4 null mice exhibit a dramatic reduction in long term plasticity at synapses between granule cell parallel fibers and Purkinje cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kim, EE., et al. (2014). J. Clin. Invest. 124(11); 5027-5036; Wang, X., et al. (2013). J. Biol. Chem. 288(3); 2040-2048). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |