Anti-Calreticulin Antibody, clone 1F16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1681 - recombinant, expressed in HEK 293 cells
Synonym: CRP55
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:100 dilution of Cat. No. ZRB1681, Anti-Calreticulin, clone 1F16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the endoplasmic reticulum.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:100 dilution of Cat. No. ZRB1681, Anti-Calreticulin, clone 1F16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the endoplasmic reticulum.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:100 dilution of Cat. No. ZRB1681, Anti-Calreticulin, clone 1F16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the endoplasmic reticulum.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from U2OS (Lane 1) and NIH3T3 (Lane 2) cells were probed with Cat. No. ZRB1681, Anti-Calreticulin, clone 1F16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Calreticulin (~58 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Calreticulin peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1681, Anti-Calreticulin, clone 1F16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Calreticulin peptide displayed a KD of 1.0 x 10-12, whereas binding to non-specific control peptide displayed a KD of 3.9 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1F16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 48.14 kDa |
| observed mol wt ~58 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_004334  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | monkey, horse, rat |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P27797 |
| Application: | Quality Control Testing Evaluated by Western Blotting in U2OS cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Calreticulin in U2OS cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Calreticulin in NIH3T3 cell lysate. Affinity Binding Assay: A representative lot of this antibody bound Calreticulin with a KD of 1.0 x 10-12 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calreticulin in U2OS cells.. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal half of human Calreticulin. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1F16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Calreticulin. It targets an epitope within 21 amino acids from the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Calreticulin (UniProt: P27797; also known as CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60) is encoded by the CALR (also known as CRTC) gene (Gene ID: 811) in human. Calreticulin is a highly conserved, monomeric, endoplasmic reticulum protein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form (aa 18-417). It serves as a calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum via the calreticulin/calnexin cycle. It consists of three distinct domains: An N-terminal lectin-like globular domain that is required for its interaction with integrins and it contains a steroid receptor-like DNA-binding site that is involved in chaperone functions; a second domain is a central proline-rich domain that forms an elongated arm-like structure and has high-affinity and low-capacity for calcium-binding and participates in its chaperone activity. The third domain is a highly acidic, localized in the C-terminal region, displays low-affinity and high-capacity for Ca2+ binding and is involved in calcium -buffering functions of calreticulin. It also has a KDEL motif at the C-terminus that is responsible for calreticulin retrieval from the Golgi apparatus to the endoplasmic reticulum. Cancer cells undergoing immunogenic cell death are reported to expose calreticulin on their surface, which promotes the uptake of dead cells by phagocytes. Mutations leading to loss-of-function of CALR gene promote oncogenesis by impairing homeostasis in healthy cells and hamper natural and therapy-driven immunosurveillance. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fucikova, J., et al. (2021). Cell Res. 31(1); 5-16; Krause, KH., and Michalak, M. (1997). Cell. 88(4); 439-443). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |