Anti-ELF5 Antibody, clone 1K15 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1655 - recombinant, expressed in HEK 293 cells
Synonym: ETS-related transcription factor Elf-5
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from U2OS (Lane 1), MDA-MB-231 (Lane 2), C6 (Lane 3), and NIH3T3 (Lane 4) cells were probed with Cat. No. ZRB1655, Anti-ELF5, clone 1K15 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ELF5 (~39 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of non-specific GST protein (Run 1) or recombinant ELF5 protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1655, Anti-ELF5, clone 1K15 ZooMAb® Rabbit Monoclonal. The binding of this antibody to ELF5 protein displayed a KD of 1.0 x 10-12, whereas binding to non-specific GST protein displayed a KD of 3.8 x 10-3.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 mg of ELF5 peptide was analyzed by ELISA using various dilutions (starting at 3 mg/mL) of Cat. No. ZRB1655, Anti-ELF5, clone 1K15 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1K15, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 31.26 kDa |
| observed mol wt ~39 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_938195  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human, rat |
| species reactivity (predicted by homology) | feline, bovine, monkey, canine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9UKW6 |
| Application: | Quality Control Testing Evaluated by Western Blotting in NIH3T3 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ELF5 in NIH3T3 cell lysate. Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ELF5 in U2OS, MDA-MB-231, and C6 cell lysates. ELISA Analysis: A representative lot of this antibody detected ELF5 peptide in ELISA application. Affinity Binding Assay:: A representative lot of this antibody bound recombinant ELF5 protein with a KD of 1.0 x 10-12 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human ETS-related transcription factor Elf-5 (ELF5). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1K15 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects ETS-related transcription factor Elf-5 (ELF5). It targets an epitope within 19 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | ETS-related transcription factor Elf-5 (UniProt: Q9UKW6; also known as E74-like factor 5, Epithelium-restricted ESE-1-related Ets factor, Epithelium-specific Ets transcription factor 2, ESE-2) is encoded by the ELF5 (also known as ESE2) gene (Gene ID: 2001) in human. The ETS transcription factors are a large family of proteins that are implicated in the control of cellular proliferation and tumorigenesis. They contain a highly conserved DNA binding domain known as the ETS domain. ELF5 is a transcriptional activator that is expressed in tissues with a high content of epithelial cells. It is highly expressed in salivary gland, mammary gland, kidney, and prostate. Its expression is not observed in hematopoietic compartments. It plays a role in regulating the later stages of keratinocytes terminal differentiation. Its ETS domain is localized to amino acids 173-254. It recognizes and binds to purine rich GGA(A/T) core motifs in the promoters and enhancers of various target genes. It also contains a PNT domain (aa 43-129) that acts as a transcriptional activator. Four isoforms of ELF5 have been described that are produced by alternative splicing. ELF5 is localized to human chromosome 11p13 15, a region that frequently undergoes loss of heterozygosity (LOH) in several types of carcinoma, including breast, kidney, and prostate carcinomas. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oettgen, P., et al. (1999). J. Biol. Chem. 274(41); 29439-29452; Zhou, J., et al. (1998). Oncogene. 17(21); 2719-2732). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |