Anti-MIF Antibody, clone 1K16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1653 - recombinant, expressed in HEK 293 cells
Synonym: Macrophage migration inhibitory factor;EC:5.3.2.1;Glycosylation-
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human prostate (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous/nuclear staining was observed in glandular cells of human prostate tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human prostate (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous/nuclear staining was observed in glandular cells of human prostate tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human prostate (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous/nuclear staining was observed in glandular cells of human prostate tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human prostate (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous/nuclear staining was observed in glandular cells of human prostate tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human prostate (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous/nuclear staining was observed in glandular cells of human prostate tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cells was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytosol and secreted form.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cells was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytosol and secreted form.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of Jurkat cells was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytosol and secreted form.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytosol and secreted form..
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytosol and secreted form..
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytosol and secreted form..
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from PC12 cells (Lane 1), THP-1 cells (Lane 2) and Human Kidney tissue (Lane 3) were probed with Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MIF (~12 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of non-specific GST protein (Run 1) or recombinant MIF protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1653, Anti-MIF, clone 1K16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to MIF protein displayed a KD of 1.0 x 10-12, whereas binding to non-specific GST protein displayed a KD of 2.6 x 10-3.
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Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1K16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 12.48 kDa |
| observed mol wt ~12 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_002406  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P14174 |
| Application: | Quality Control Testing Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MIF in PC12 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MIF in lysates from THP-1 cells and Human Kidney tissue. Affinity Binding Assay: A representative lot of this antibody bound MIF with a KD of 1.0 x 10-12 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MIF in Jurkat and RAW264.7 cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MIF in human prostate tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | His-tagged full-length recombinant human Macrophage migration inhibitory factor (MIF). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1K16 is a Rabbit recombinant monoclonal antibody that specifically detects Macrophage migration inhibitory factor (MIF). |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Macrophage migration inhibitory factor (UniProt: P14174; also known as EC:5.3.2.1, MIF, Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase) is encoded by the MIF (also known as GLIF, MMIF) gene (Gene ID: 4282) in human. MIF is a pro-inflammatory cytokine that is constitutively expressed by a broad spectrum of cells and tissues, including monocytes and macrophages. It is rapidly released after exposure to microbial products, such as LPS, and pro-inflammatory mediators. It lacks a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Following its release, it induces pro-inflammatory and immune responses. MIF is involved in the innate immune response to bacterial pathogens and serves as a mediator in regulating the function of macrophages in host defense and counteracts the anti-inflammatory activity of glucocorticoids. Serum levels of MIF are significantly elevated in subjects with severe sepsis or septic shock and high levels of MIF are correlated with low survival rate. Multiple mechanisms of action have been attributed to MIF. It can bind to CD74 and phosphorylate and activate ERK1/ERK2 mitogen-activated protein kinase pathway. It also binds to JUN activation domain-binding protein 1 (JAB1) and prevents JAB-1-induced activation of Jun and JAB-1 induced degradation of Kip1, which leads to cell cycle arrest and apoptosis. Mutations in MIF gene are known to cause systemic juvenile Rheumatoid arthritis, an inflammatory articular disorder that begins before the age of 16 and cause severs extraarticular features and fatal complications. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Emonts, M., et al. (2007). Clin. Infect. Dis. 44(10); 1321-1328; Shi, X., et al. (2006); Immunity. 25(4); 595-606; Calandra, T., and Roger, T. (2003). Nat. Rev. Immunol. 3(10); 791-800). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |