Anti-Lubricin/PRG4 Antibody, clone 1C20 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1643 - recombinant, expressed in HEK 293 cells
Synonym: Proteoglycan 4
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Mouse Cartilage (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1643, Anti-Lubricin/PRG4, clone 1C20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in femoral chondrocytes of mouse knee joint tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Mouse Cartilage (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1643, Anti-Lubricin/PRG4, clone 1C20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in femoral chondrocytes of mouse knee joint tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Mouse Cartilage (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1643, Anti-Lubricin/PRG4, clone 1C20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in femoral chondrocytes of mouse knee joint tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Recombinant human PRG4 protein was probed with Cat. No. ZRB1643, Anti-Lubricin/PRG4, clone 1C20 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Lubricin/PRG4 (~30 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Lubricin/PRG4 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1643, Anti-Lubricin/PRG4, clone 1C20 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Lubricin/PRG4 peptide displayed a KD of 1.6 x 10-7, whereas binding to non-specific control peptide displayed a KD of 1.6 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1C20, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 151.06 kDa |
| observed mol wt ~30 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_005798  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q92954 |
| Application: | Quality Control Testing Evaluated by Western Blotting with recombinant human PRG4 protein. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human Lubricin/PRG4. Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Lubricin/PRG4 in mouse cartilage tissue sections. Affinity Binding Assay: A representative lot of this antibody bound Lubricin/PRG4 with a KD of 1.6 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Lubricin (Proteoglycan 4). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1C20 is a ZooMAb® Rabbit recombinant monoclonal antibody that detects Lubricin (Proteoglycan 4). It targets an epitope within 24 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Proteoglycan 4 (UniProt: Q92954; also known as Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan) is encoded by the PRG4 (also known as MSF, SZP) gene (Gene ID: 10216) in human. Proteoglycan 4 is a heavily O-glycosylated, secreted, mucin-like protein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form (aa 25-1404) that can be further cleaved into a C-terminal part (aa 1307-1404). It is highly expressed in synovial tissue, cartilage, and liver and is weakly expressed in heart and lung. It is reported to play a role in boundary lubrication within articulating joints and prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Human lubricin contains two mucin-like domains. The first mucin domain contains multiple copies of the peptide motif KEPAPTTT, with threonine residues that are critical for O-linked GalNAc-Gal-NeuAc sidechains. The second mucin-like domain is also threonine rich but does not contain the KEPAPTTT motif. It also contains a hemopexin-like (PEX) domain at its C-terminal that is suggested to be essential for extracellular matrix binding. Lack of proteoglycan 4 expression leads to premature joint failure. Its low concentration in synovial fluid following an injury is associated with increased cartilage damage and may play a role in development of osteoarthritis. Proteoglycan 4 is reported to reduce neuroinflammation and protect the blood-brain barrier after traumatic brain injury. It restores blood-brain barrier function and prevents post-traumatic loss of tight junction protein claudin 5 and reduces neuronal death. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bennett, M., et al. (2021). J. Neurotrauma. 38(4); 385-398; Iqbal, SM., et al. (2016). Sci. Rep. 6; Article 18910). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |