Anti-Cathepsin L1 Antibody, clone 1G2 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1636 - recombinant, expressed in HEK 293 cells
Synonym: EC:3.4.22.15;Major excreted protein;MEP
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in subsets of tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in subsets of tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in subsets of tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in subsets of tubules of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A and B) and Negative control (no primary) (C and D) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic staining was observed in subsets of tubules of human kidney tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cells was performed using a 1:100 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cells was performed using a 1:100 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cells was performed using a 1:100 dilution of Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology PC12 (Left lane) and A549 (Right Lane) cell lysates were probed with Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cathepsin L1 (~25, 32, and 37 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Cathepsin L1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1636, Anti-Cathepsin L1, clone 1G2 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Cathepsin L1 peptide displayed a KD of 4.1 x 10-7, whereas binding to non-specific control peptide displayed a KD of 1.2 x 10-3.
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12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1G2, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 37.56 kDa |
| observed mol wt ~25, 32, 37 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_001903.1  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, rat |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P07711 |
| Application: | Quality Control Testing Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cathepsin L1 in PC12 cell lysate. Tested applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cathepsin L1 in A549 cell lysate. Affinity Binding Assay: A representative lot of this antibody bound Cathepsin L1 peptide with a KD of 4.1 x 10-7 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cathepsin L1 in A549 cells Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cathepsin L1 in human kidney tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Cathepsin L. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1G2 is a Rabbit recombinant monoclonal antibody that specifically detects Cathepsin L. It targets an epitope within 14 amino acids from the C-terminal half. It detects procathepsin L, mature Cathepsin L, and Cathepsin L heavy chain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Procathepsin L (UniProt: P07711; also known as EC:3.4.22.15, Cathepsin L, Major excreted protein, MEP) is encoded by the CTSL (also known as CTSL1) gene (Gene ID: 1514) in human. Cathepsin L is a lysosomal protease that is synthesized as a procathepsin with a signal peptide (aa 1-17) and two propeptides (aa 18-113 activation peptide; and 289-291) that are cleaved off to produce mature active enzyme. After the removal of these peptides, cathepsin can exist in three forms: cathepsin L (aa 114-333), cathepsin L heavy chain (aa 114-288) and cathepsin L light chain (aa 292-333). The heavy and light chains are connected by disulfide bonds. Active cathepsin L is a thiol protease with endopeptidase activity and preferentially cleaves peptide bonds with aromatic residues in P2 position and hydrophobic residues in P3 position. It is catalytically active in the pH range 3 to 6.5. It participates in overall degradation of proteins in lysosomes and plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. It is also Involved in the solubilization of cross-linked thyroglobulin and in subsequent release of thyroxine (T4) by limited proteolysis of thyroglobulin in the thyroid follicle lumen. Cathepsin L has been implicated in several pathological conditions, including invasion and tumor metastasis, atherosclerosis, inflammation, and degradation of extracellular matrix that is reported to be an important process in entry of SARS-CoV-2 into host cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gomes, CP., et al. (2020). Front. Cell. Infect. Microbiol. 10; 589505; Turk, V., et. al. (2012). Biochim. Biophys. Acta. 1824(1); 68-88). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |