Anti-PLD2 Antibody, clone 6F13 ZooMAb^® Rabbit Monoclonal

SIGMA/ZRB1629 - recombinant, expressed in HEK 293 cells

Synonym: Choline phosphatase 2; EC:3.1.4.4; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2; Phospholipase D2; hPLD2

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-ZRB1629-25UL	25 µL	$223.00 1/EA	Add To Favorites
45-ZRB1629-4X25UL	25 µL	$460.00 1/EA	Add To Favorites

Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was primarily observed in acinar cells of human pancreas tissue sections.

Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was also observed in tubules and glomeruli of human kidney tissue sections.

Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line was performed using a 1:100 dilution of Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.

Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A431 cell line was performed using a 1:100 dilution of Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.

Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NIH3T3 cell line was performed using a 1:100 dilution of Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.

Western Blotting Enhanced Validation - Recombinant Antibody Technology NIH3T3 cell lysate was probed with Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PLD2 (~105 kDa).

Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of of non-specific control peptide (Run 1) or PLD2 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal. The binding of this antibody to PLD2 peptide displayed a KD value of 1.7 x 10-6 whereas its binding to non-specific peptide displayed a KD of 3.6 x 10-3.

Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million NIH3T3 cells was performed using 1 μg of Cat. No. ZRB1629, Anti-PLD2, clone 6F13 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-RabbitIgG secondary antibody.

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified antibody
biological source	rabbit
clone	recombinant monoclonal
conjugate	unconjugated
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
form	lyophilized
greener alternative category	Aligned
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
isotype	IgG
mol wt	calculated mol wt 105.98 kDa
	observed mol wt ~105 kDa
packaging	antibody small pack of 25 μL
product line	ZooMAb^® learn more
Quality Level	200
recombinant	expressed in HEK 293 cells
shipped in	ambient
species reactivity	human, mouse
storage temp.	2-8°C
technique(s)	affinity binding assay: suitable
	flow cytometry: suitable
	immunocytochemistry: suitable
	immunohistochemistry: suitable
	western blot: suitable
UniProt accession no.	O14939

Application:	Quality Control Testing Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PLD2 in NIH3T3 cell lysate.Tested applicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PLD2 in HeLa, A431, and d NIH 3T3 cells..Flow Cytometry Analysis: 1 μg from a representative lot detected PLD2 in one million NIH3T3 cells.Affinity Binding Assay: A representative lot of this antibody bound PLD2 peptide with a KD of 1.7 x 10-6 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PLD2 in human pancreas and human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Application:	Anti-PLD2, clone 6F13 ZooMAb, Cat. No. ZRB1629, is a recombinant Rabbit monoclonal antibody that detects Phospholipase D2 and is tested in Affinity Binding Assay, Flow Cytometry and Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
General description:	ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here.
Immunogen:	KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Phospholipase D2.
Legal Information:	ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution:	300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity:	Clone 6F13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Phospholipase D2. It targets an epitope within 21 amino acids from the N-terminal region.
Storage and Stability:	Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description:	Phospholipase D2 (UniProt: O14939; also known as EC:3.1.4.4, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2) is encoded by the PLD2 gene (Gene ID: 5338) in human. PLD2 is a peripheral membrane protein that displays ubiquitous expression and plays a role in cell membrane lipid reorganization and serves as a key cell signaling protein. It is activated by a wide variety of G-protein coupled receptors and receptor tyrosine kinases in response to a wide variety of agonists. It hydrolyzes the membrane phospholipid phosphatidylcholine to choline and the lipid messenger phosphatidic acid (PA). PA can act as a central lipid second messenger for various signaling pathways and participates in many physiological processes, including cell proliferation, migration, and cytoskeletal organization. PA is also involved in the activation of Ras‐GTPase by recruiting its exchange factor Sos or Rac1to the plasma membrane. PLD2 is reported to positively regulate proliferation of CD8+ T cells that play a critical role in cancer cell elimination and suppression of tumor progression. PLD2 is reported to degrade HIF-1 and thereby reduces VEGF secretion by endothelial cells that results in reduction of tumor neovascularization and growth. PLD2 has PX and PH domains at its regulatory N‐terminus that are putatively responsible for its GEF activity. It also has two conserved HKD domains are responsible for the lipase activity and conversion of PC into PA. Its catalytic region is localized within amino acids 441-788. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bich, VNT., et al. (2018). Sci. Rep. 8; 6283; Frohman, MA. (2015). Trends Pharmacol. Sci. 36(3); 137-144; Park, MH., et al. (2015). Exp. Mol. Med. 47; 196; Gomez-Cambronero, J. (2012). J. Leukocyte Biol. 92(3); 461-467).

WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352203

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