Anti-S100-B Antibody, clone 8F20 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1628 - recombinant, expressed in HEK 293 cells
Synonym: Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Varying degrees of cytoplasmic/membranous/nuclear staining were observed in both granular and molecular layers, as well as Purkinje and glial cells.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Varying degrees of cytoplasmic/membranous/nuclear staining were observed in both granular and molecular layers, as well as Purkinje and glial cells.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Varying degrees of cytoplasmic/membranous/nuclear staining were observed in both granular and molecular layers, as well as Purkinje and glial cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A375 cells was performed using a 1:100 dilution of Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains both nuclear and cytoplasmic.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A375 cells was performed using a 1:100 dilution of Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains both nuclear and cytoplasmic.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A375 cells was performed using a 1:100 dilution of Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains both nuclear and cytoplasmic.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from Mouse Spinal Cord (Lane 1), A375 cells (Lane 2), Rat Brain (Lane 3), and Human Brain (Lane 4) were probed with Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates S100-B (~10 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of non-specific GST protein (Run 1) or S100-B protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal. The binding of this antibody to S100-B protein displayed a KD value of 3.2 x 10-7 whereas its binding to non-specific GST protein displayed a KD of 1.7 x 10-3.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million C6 cells was performed using 1 μg per million of Cat. No. ZRB1628, Anti-S100-B, clone 8F20 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit IgG secondary.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| biological source | rabbit |
| clone | recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 10.71 kDa |
| observed mol wt ~10 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P04271  |
| Application: | Quality Control Testing Western Blotting Analysis: A 1:10,000 dilution of this antibody detected S100-B in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected S100-B in mouse spinal cord, human brain, and A375 lysates.Immunohistochemis |
| Application: | Anti-S100-B, clone 8F20 ZooMAb, Cat. No. ZRB1628, is a recombinant Rabbit monoclonal antibody that specifically targets S100-B and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged full-length recombinant human S100-B protein. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 8F20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects S100-B. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Protein S100-B (UniProt: P04271; also known as S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B) is encoded by the S100B gene (Gene ID: 6285) in human. S100-B is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. It exists in both heterodimeric and homodimeric forms. Existence of dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain has been reported and dimerization seems to be essential for its biological activities. S100-B has been suggested to play a part in a variety of cellular processes, primarily via binding to key synaptic proteins and inhibiting their phosphorylation. The extracellular form of S100-B, secreted by astrocytes, is physiologically involved in the development and maintenance of CNS homeostasis. S100-B effects are closely dependent on its cellular levels. At nanomolar concentrations it is shown to enhance survival of neurons during development and stimulate neurite outgrowth in cerebral cortex neurons. It also reduces the loss of mitochondrial function and cell death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Donato, R. (2001). Int. J. Biochem. Cell Biol 33(7); 637-668; Rezaei, O., et al. (2017). Iran J. Neurol. 16(2); 83-89). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |