Anti-PKCδ Antibody, clone 1C13 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1623 - recombinant, expressed in HEK 293 cells
Synonym: EC:2.7.11.13; Protein kinase C delta type; nPKC-delta
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1623, Anti-PKCδ, clone 1C13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic/membranous staining was observed in Purkinje cells, as well as neuronal processes in granular and molecular layers of human cerebellum sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1623, Anti-PKCδ, clone 1C13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic/membranous staining was observed in Purkinje cells, as well as neuronal processes in granular and molecular layers of human cerebellum sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1623, Anti-PKCδ, clone 1C13 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Punctate cytoplasmic/membranous staining was observed in Purkinje cells, as well as neuronal processes in granular and molecular layers of human cerebellum sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1623, Anti- PKCd , clone 1C13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1623, Anti- PKCd , clone 1C13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1623, Anti- PKCd , clone 1C13 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from Rat brain (Lane 1), Mouse brain (Lane 2), A431 cells (Lane 3), and SH-SY5Y cells (Lane 4) were probed with Cat. No. ZRB1623, Anti- PKCd, clone 1C13 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PKCd (~77 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of control peptide (Run 1) or PKCd peptide peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1623-25UL, Anti-PKCδ, clone 1C13 ZooMAb® Rabbit Monoclonal. The binding of this antibody to PKCd peptide displayed a KD value of 2.2 x 10-8 whereas its binding to control peptide displayed a KD of 8.1 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1C13, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 77.51 kDa |
| observed mol wt ~77 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q05655  |
| Application: | Anti-PKCd, clone 1C13 ZooMAb, Cat. No. ZRB1623, is a recombinant Rabbit monoclonal antibody that specifically targets PKCd and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Integrin -5 in HT-1080 and NIH3T3 cell lysates. Flow Cytometry Analysis: 1 μg from a representative lot detected Integrin -5 in one million HelL cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Integrin -5 in human placenta tissue sections. Affinity Binding Assay: A representative lot of this antibody bound Integrin a-5 peptide with a KD of 2.5 x 10-8 in an affinity binding assay. Immunocytochemistry Analysis: A 1:1,0000 dilution from a representative lot detected Integrin -5 in HeLa cell line, A431 cell line, HUVEC cell line and NIH 3T3 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal AGC kinase domain of human protein kinase C delta ( PKCd). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1C13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects PKCd It targets an epitope within 18 amino acids from the C-terminal, AGC kinase domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Protein kinase Cd (UniProt: Q05655; also known as EC:2.7.11.13, nPKC-delta) is encoded by the PRKCD gene (Gene ID: 5580) in human. PKC d is a ubiquitous serine/threonine kinase involved in signal transduction associated with cell proliferation, differentiation, and apoptosis. It is a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent novel PKC enzyme that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death. It is shown to negatively regulate B cell proliferation and is also involved in self-antigen induced B cell tolerance induction. Upon DNA damage, PKCd activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In the case of ER stress or DNA damage-induced apoptosis, it can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol it can trigger apoptosis by activating MAPK11 or MAPK14 and inhibiting Akt1 and decreasing the level of XIAP. Phosphorylation at three specific sites: threonine 507, serine 645, and serine 664 is required for its full activation. PKCd is proteolytically cleaved into a catalytic subunit (aa 330-676) and a regulatory subunit (aa 1-329) by caspase-3 during apoptosis which results in kinase activation. As a result of this cleavage the pseudosubstrate motif in the regulatory subunit is released from the substrate recognition site of the catalytic subunit, which enables its activation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |