Anti-IFNγ Antibody, clone 5K15 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1620 - recombinant, expressed in HEK 293 cells
Synonym: IFN-gamma; Immune interferon; Interferon gamma
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NK-92 cells treated with BFA were performed using a 1:1,000 dilution of Cat. No. ZRB1620, Anti-IFN, clone 5K15 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm of stimmed and treated cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NK-92 cells treated with BFA were performed using a 1:1,000 dilution of Cat. No. ZRB1620, Anti-IFN, clone 5K15 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm of stimmed and treated cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NK-92 cells treated with BFA were performed using a 1:1,000 dilution of Cat. No. ZRB1620, Anti-IFN, clone 5K15 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm of stimmed and treated cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NK-92 cells stimmulated and treated with BFA were performed using a 1:1,000 dilution of Cat. No. ZRB1620, Anti-IFN, clone 5K15 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm of stimmed and treated cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NK-92 cells stimmulated and treated with BFA were performed using a 1:1,000 dilution of Cat. No. ZRB1620, Anti-IFN, clone 5K15 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm of stimmed and treated cells.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NK-92 cells stimmulated and treated with BFA were performed using a 1:1,000 dilution of Cat. No. ZRB1620, Anti-IFN, clone 5K15 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody conjugated to Cy3. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm of stimmed and treated cells.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Recombinant IFN-g protein was probed with Cat. No. ZRB1620, Anti-IFN-g, clone 5K15 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IFN-g (~15 kDa for recombinant IFN-g).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of non-specific GST protein (Run 1) or IFN-g protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1620, Anti-IFN-g, clone 5K15 ZooMAb® Rabbit Monoclonal. The binding of this antibody to IFN-g displayed a KD value of 2.3 x 10-8 whereas its binding to non-specific GST protein displayed a KD of 2.7 x 10-3.
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million Human PBMC was performed using 1 μg of Cat. No. ZRB1620, Anti-IFNγ, clone 5K15 ZooMAb® Rabbit Monoclonal. Histogram represents the gated R3 population shown in the dot plot.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| biological source | rabbit |
| clone | recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 19.35 kDa |
| observed mol wt ~15 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P01579  |
| Application: | Quality Control Testing Western Blotting Analysis (WB): Analysis: A 1:1,000 dilution of this antibody detected human IFN-g protein.Tested applications Western Blotting Analysis (WB): A 1:1000 Dilution of this antibody detected IFN- g in NK-92 cell lysate. Flow Cytometry Analysis: 1 μg from a representative lot detected IFN- in one million HuPBMC.Immunocytochemistr |
| Application: | Anti-IFN-g, clone 5K15 ZooMAb, Cat. No. ZRB1620, is a recombinant Rabbit monoclonal antibody that specifically targets IFN-g and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | Full-length mature recombinant human Interferon- g (without signal and propeptide). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 5K15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Interferon- g. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Interferon- g (UniProt: P01579; also known as IFN-gamma, IFN- g, Immune interferon) is encoded by the IFNG gene (Gene ID: 3458) in human. IFN-g is a pleiotropic molecule with antiproliferative, pro-apoptotic, and antitumor properties. It is a type II homodimeric interferon that is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 162-166) that are subsequently cleaved off to produce the mature form. It is secreted predominantly by activated lymphocytes such as CD4 T helper type 1, CD8 cytotoxic T cells, g T cells, and natural killer cells, and antigen-presenting cells. Its expression is induced by mitogens and cytokines. IFN- g signaling coordinates several biological responses involved in host defense and immune surveillance, establishment of adaptive immunity, and the regulation of inflammation. Its diverse biological activities are initiated by IFN- g -mediated aggregation of at least two different cell surface receptors: IFN g R1 and IFN g R2. IFN-g is reported to activate macrophages toward a pro-inflammatory profile increasing their phagocytic ability and enhancing their microbial killing activity. It is also shown to upregulate cell surface MHC class II on antigen presenting cells, thus promoting peptide-specific activation of CD4 T cells. Mutations in IFNG gene are known to cause aplastic anemia that is characterized by failure of bone marrow to produce adequate number of peripheral blood elements leading to peripheral pancytopenia and hypoplasia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Castro, F., et al. (2018). Front. Immunol. 9; 847). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |