Anti-IKKβ Antibody, clone 2N22 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1595 - recombinant, expressed in HEK 293 cells
Synonym: Inhibitor of nuclear factor kappa-B kinase subunit beta;EC:2.7.11.10;I-
Product Type: Chemical
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from A549 (Lane 1), HepG2 (Lane 2), L6 (Lane 3) and NIH3T3 (Lane 4) cells were probed with Cat. No. ZRB1595, Anti-IKKβ, clone 2N22 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IKKβ (~90 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or IKKb peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1595, Anti-IKKβ, clone 2N22 ZooMAb® Rabbit Monoclonal. The binding of this antibody to IKKb peptide displayed a KD of 7.9 x 10-8, whereas binding to non-specific control peptide displayed a KD of 2.5 x 10-5.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 mg of human recombinant IKKb protein was analyzed by ELISA using various dilutions (starting at 3 mg/mL) of Cat. No. ZRB1595, Anti-IKKβ, clone 2N22 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2N22, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 86.56 kDa |
| observed mol wt ~90 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_001177649  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | bovine, monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | O14920 |
| Application: | Quality Control Testing Evaluated by Western Blotting in A549 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IKKβ in A549 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IKKβ in HepG2, L6, and NIH3T3 cell lysates. Affinity Binding Assay: A representative lot of this antibody bound IKKb with a KD of 7.9 x 10-8 in an affinity binding assay. Enzyme Immunoassay (ELISA) Analysis: Various dilutions of this antibody detected IKKβ in ELISA application. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human IKKb. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2N22 is a Rabbit recombinant monoclonal antibody that specifically detects IKKb. It targets an epitope within 24 amino acids from the N-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Inhibitor of nuclear factor kappa-B kinase subunit beta (UniProt: O14920; also known as EC:2.7.11.10, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, Serine/threonine protein kinase IKBKB) is encoded by the IKBKB (also known as IKKB) gene (Gene ID: 3551) in human. The IKK complex is composed of three subunits, IKKa , IKKb, and IKKg (NEMO). While IKKa and IKKb are highly similar catalytic subunits and can phosphorylate IkB, IKKg is the regulatory subunit. IKKb is a serine kinase that plays an essential role in the NF-kB signaling pathway. It acts as a part of the canonical IKK complex in the conventional pathway of NF-kB activation. Its protein kinase domain is localized in amino acids 15-300. It phosphorylates inhibitors of NF-kB (IkB) on two critical serine residues that allow polyubiquitination of these inhibitors and their subsequent degradation by the proteasome. This allows free NF-kB to translocate into the nucleus and activate the transcription of genes involved in immune response, growth control, and protection against apoptosis. Upon cytokine stimulation, IKKb is phosphorylated on serine 177 and serine 181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and protein kinase Cz that enhances its activity. Once activated, it undergoes autophosphorylation on the C-terminal serine cluster, which reduces its activity and prevents prolonged activation of the inflammatory response. Mutations in IKBKB gene are known to cause life threatening immunodeficiency that develops in in infancy and increases susceptibility to bacterial, fungal, and viral infections. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, ZW., et al. (1999). J. Exp. Med. 189(11); 1839-1845). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |