Anti-CCPG1 Antibody, clone 1F15 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1582 - recombinant, expressed in HEK 293 cells
Synonym: Cell cycle progression protein 1; Cell cycle progression restoration protein 8
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human small intestine (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells and lamina propria of human small intestine tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human small intestine (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells and lamina propria of human small intestine tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human small intestine (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells and lamina propria of human small intestine tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SK-MEl-28 cells was performed using a 1:100 dilution of Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SK-MEl-28 cells was performed using a 1:100 dilution of Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SK-MEl-28 cells was performed using a 1:100 dilution of Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Recombinant CCPG1 (Left image) and A549 cell lysate (Right image) were probed with Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates CCPG1 (~ 34 kDa for recombinant fragment of CCPG1; ~90 kDa in A549 cell lysate).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of non-specific GST protein (Run 1) or recombinant CCPG1 protein (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1582, Anti-CCPG1, clone 1F15 ZooMAb® Rabbit Monoclonal. The binding of this antibody to recombinant CCPG1 protein displayed a KD value of 3.9 x 10-7 whereas its binding to non-specific GST protein displayed a KD of 4.2 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1F15, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 87.34 kDa |
| observed mol wt ~34 kDa (recombinant fragment) | |
| observed mol wt ~90 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9ULG6  |
| Application: | Anti-CCPG1, clone 1F15 ZooMAb, Cat. No. ZRB1582, is a recombinant Rabbit monoclonal antibody that specifically targets CCPG1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CCPG1 in A549 cell lysate. Affinity Binding Assay: A representative lot of this antibody bound recombinant CCPG1 with a KD of 3.9 x 10-7 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CCPG1 in SK-MEl-28 cells. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CCPG1 in human small intestine tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged recombinant fragment corresponding to 250 amino acids from the C-terminal, lumenal domain of human Cell cycle progression protein 1 (CCPG1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1F15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Cell cycle progression protein 1 (CCPG1). It targets an epitope within the lumenal domain in the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Cell cycle progression protein 1 (UniProt: Q9ULG6; also known as Cell cycle progression restoration protein 8, CCPG1) is encoded by the CCPG1 (also known as CCP8, CPR8, KIAA1254) gene (Gene ID: 9236) in human. CCPG1 is a single-pass type II membrane protein that acts as an assembly platform for Rho protein signaling complexes. It is reported to mediate recruitment of endogenous Src kinase into Dbs-containing complexes and interacts with the Rho family member Cdc42. It limits guanine nucleotide exchange activity of MCF2L toward RHOA, which results in an inhibition of both its transcriptional activation ability and its transforming activity. However, it does not inhibit activity of MCF2L toward CDC42, or activity of MCF2 toward either RHOA or CDC42. CCPG1 also serves as an ER stress-inducible endoplasmic reticulum (ER)-phagy cargo receptor in mammals. It is shown to bind directly to ATG8 and FIP200 via distinct peptide motifs. It is reported to offer protection against ER luminal protein aggregation and consequent unfolded protein response hyperactivation and tissue injury of the exocrine pancreas. CCPG1 contains a cytoplasmic domain (aa 1-217), a transmembrane domain (aa 218-238), and a luminal domain (aa 239-757). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Smith, MD., et al. (2018). Dev. Cell. 44(2); 217-232; Kostenko EV., et al. (2006). Mol. Cell. Biol. 26(23); 8964-8975). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |