Anti-ISL-1 Antibody, clone 1B11 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1579 - recombinant, expressed in HEK 293 cells
Synonym: Insulin gene enhancer protein ISL-1;Islet-1
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas tissue sections (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear/nuclear membranous staining was observed in subsets of both exocrine & endocrine cells, as well as ductal cells of human pancreatic tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas tissue sections (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear/nuclear membranous staining was observed in subsets of both exocrine & endocrine cells, as well as ductal cells of human pancreatic tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human pancreas tissue sections (A) and Negative control (no primary) (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Nuclear/nuclear membranous staining was observed in subsets of both exocrine & endocrine cells, as well as ductal cells of human pancreatic tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells (A and B) and negative control (no primary) (C and D) cells were performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells (A and B) and negative control (no primary) (C and D) cells were performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells (A and B) and negative control (no primary) (C and D) cells were performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells (A and B) and negative control (no primary) (C and D) cells were performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells (A and B) and negative control (no primary) (C and D) cells were performed using a 1:1,000 dilution of Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology SH-SY5Y cell lysate was probed with Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ISL-1 (~42 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or ISL-1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1579, Anti-ISL-1, clone 1B11 ZooMAb® Rabbit Monoclonal. The binding of this antibody to ISL-1 peptide displayed a KD of 5.6 x 10-8, whereas binding to non-specific control peptide displayed a KD of 1.6 x 10-4.
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12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1B11, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 39.04 kDa |
| observed mol wt ~42 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_002193  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | canine, bovine, rat, monkey, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P61371 |
| Application: | Quality Control Testing Evaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected ISL-1 in human pancreas tissue sections. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ISL-1 in SH-SY5Y cell lysate. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ISL-1 in SH-SY5Y cells. Affinity Binding Assay: A representative lot of this antibody bound ISL-1 peptide with a KD of 5.6 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 21 amino acids from the LIM binding domain in the C-terminal region of human Insulin gene enhancer protein ISL-1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1B11 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Insulin gene enhancer protein ISL-1. It targets an epitope within 21 amino acids within the LIM binding domain in the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Insulin gene enhancer protein ISL-1 (UniProt: P61371; also known as Islet-1, ISL-1) is encoded by the ISL1 gene (Gene ID: 3670) in human. ISL-1 is a member of the LIM homeodomain family of transcription factors that contain two tandemly repeated cysteine/histidine-rich LIM domains that are involved in protein-protein interactions. It is a DNA-binding transcriptional activator that recognizes and binds to the consensus octamer binding site 5′-ATAATTAA-3′ in promoter of target genes. Its expression is observed in in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. It is shown to play a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It is also involved in the specification of motor neurons in cooperation with LHX3 and LDB1. ISL-1 is also established as an important stem cell marker for cardiac progenitor cells in the developing heart. It is reported to bind to compacted chromatin and works in concert with the Brg1-Baf60c-based SWI/SNF complex to promote cardiac lineage-specific alterations in the chromatin landscape of genes that function in cardiac progenitors and cardiomyocyte structural genes. It is also expressed in a subpopulation of striatal progenitor cells that give rise to striatal neurons that belong to the striatonigral pathway. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gao, R., et al. (2019). Cell Res. 29(6); 486-501; Ehrman, LA., et al. (2013). Proc. Natl. Acad. Sci. USA. 110(42); E40276-E4035). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |