Anti-Insulin Receptor β Antibody, clone 1J16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1570 - recombinant, expressed in HEK 293 cells
Synonym: EC:2.7.10.1;IR;CD220
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in tubules & glomeruli of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in tubules & glomeruli of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in tubules & glomeruli of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in tubules & glomeruli of human kidney tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human kidney (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in tubules & glomeruli of human kidney tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:1,000 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane and cytosol.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:1,000 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane and cytosol.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:1,000 dilution of Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the plasma membrane and cytosol.
Western Blotting Enhanced Validation - Recombinant Antibody Technology NIH3T3 cells (Left Lane) and Rat Liver tissue (Right Lane) lysates were probed with Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor β (~110 kDa).
Western Blotting Enhanced Validation - Recombinant Antibody Technology U2OS cell lysate (Left Lane) and human kidney tissue lysate (Right Lane) were probed with Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor β (~110 kDa).
Western Blotting Enhanced Validation - Recombinant Antibody Technology Insulin Receptor β recombinant protein was probed with Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor β (~65 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or Insulin Receptor b peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1570, Anti-Insulin Receptor β, clone 1J16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Insulin Receptor beta peptide displayed a KD of 2.2 x 10-7, whereas binding to non-specific control peptide displayed a KD of 2.4 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1J16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 156.33 kDa |
| observed mol wt ~110 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_000199  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P06213 |
| Application: | Quality Control Testing Evaluated by Western Blotting in rat liver tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Insulin Receptor β in rat liver tissue lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant human Insulin Receptor β and Insulin Receptor b in lysates from Human Kidney and NIH3T3 and U2OS cells. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Insulin Receptor β in U2OS cells. Affinity Binding Assay: A representative lot of this antibody bound Insulin Receptor b with a KD of 2.2 x 10-7 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Insulin Receptor β in human kidney tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal, cytoplasmic domain of human Insulin receptor b-subunit. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1J16 is a Rabbit recombinant monoclonal antibody that specifically detects Insulin receptor. It targets an epitope within 17 amino acids from the C-terminal cytoplasmic domain of the b-subunit. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Insulin receptor (UniProt: P06213; also known as EC:2.7.10.1, IR, CD220) is encoded by the INSR gene (Gene ID: 3643) in human. Insulin is the major hormone that counters the concerted action of a number of hyperglycemia generating and enhances glucose uptake in muscle and adipose tissue, and reduces gluconeogenesis and lipolysis. Its actions are mediated via binding to the Insulin receptor. Insulin receptor is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form. It is a tetrameric receptor composed of two a and two β chains linked by disulfide bonds. The a chains carry the insulin-binding region, while the β chains carry the kinase domain. Upon insulin binding, the receptor undergoes autophosphorylation that activates its kinase activity localized in the β subunit. The tyrosine kinase activity then leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. These phosphorylated proteins serve as docking proteins for other signaling proteins that contain SH2 domains that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Upon insulin binding the receptor undergoes autophosphorylation that activates its kinase activity localized in the β subunit. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |