Anti-Caspase-1 p10 Antibody, clone 9H11 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1563 - recombinant, expressed in HEK 293 cells
Synonym: CASP-1; Caspase-1; EC:3.4.22.36; ICE; IL-1 beta-converting enzyme; IL-1BC; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1563, Anti-Caspase-1 p10, clone 9H11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in germinal centers of human tonsil sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1563, Anti-Caspase-1 p10, clone 9H11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in germinal centers of human tonsil sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1563, Anti-Caspase-1 p10, clone 9H11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in germinal centers of human tonsil sections.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from untreated Raw 264.7 cells (Lane 1), Raw 264.7 cells treated with LPS (Lane 2), Untreated L929 cells (Lane 3), L929 cells treated with LPS (Lane 4), untreated THP-1 cells (Lane 5), and THP-1 cells treated with TPS/LPS (Lane 6) were probed with Cat. No. ZRB1563, Anti-Caspase-1 p10, clone 9H11 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Caspase-1 p10 (~10 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 mg/mL of Caspase-1 p10 peptide (Run 1) or non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1563, Anti-Caspase-1 p10, clone 9H11 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Caspase-1 p10 peptide displayed a KD value of 2.8 x 10-9 whereas its binding to non-specific control peptide displayed a KD of 1.2 x 10-4.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 9H11, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 45.16 kDa (for whole Caspase-1. ) |
| observed mol wt ~10 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P29466  |
| Application: | Anti-Caspase-1 p10, clone 9H11 ZooMAb, Cat. No. ZRB1563, is a Rabbit monoclonal antibody that specifically targets Caspase-1 p10 and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Caspase-1 p10 in lysates from L929 and THP-1 cells treated with Lipopolysaccharide (LPS). Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Caspase-1 p10 in human tonsil tissue sections. Affinity Binding Assay: A representative lot of this antibody bound Caspase-1 p10 peptide with a KD of 2.8 x 10-9 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of p10 subunit of human Caspase-1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 9H11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects p10 subunit of Caspase-1. It targets an epitope within 17 amino acids from the C-terminal region of the p10 subunit of caspase-1. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Caspase-1 (UniProt: P29466; also known as EC:3.4.22.36; CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45) is encoded by the CASP1 (also known as IL1BC, IL1BCE) gene (Gene ID: 834) in human. Caspases are secreted as latent single chain zymogens, which are further processed to create active caspases. Caspases are secreted as latent single chain zymogens, which are further processed to create active caspases. The zymogen form of caspase-1 has an N-terminal propeptide (aa 1-119) and another propeptide (aa 298-316), which are cleaved off to generate p20 (aa 120-297) and p10 (aa 317-404) subunits. Its CARD domain is localized in amino acids 1-91. The two subunits (p20 an p10) heterotetramerize to form two anti-parallel arranged heterodimers. Caspase-1 is a thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine that is involved in a variety of inflammatory processes. It is a component of the inflammasome protein complex. It has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |