Anti-Dopamine Transporter Antibody, clone 1D2 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1525 - recombinant, expressed in HEK 293 cells
Synonym: DA transporter; DAT; Sodium-dependent dopamine transporter; Solute carrier family 6 member 3
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse hippocampus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 ilution of Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in neuronal projections of mouse hippocampus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse hippocampus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 ilution of Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in neuronal projections of mouse hippocampus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) mouse hippocampus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 ilution of Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in neuronal projections of mouse hippocampus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat midbrain (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 ilution of Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in neuronal projections of rat midbrain tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat midbrain (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 ilution of Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in neuronal projections of rat midbrain tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) rat midbrain (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 ilution of Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous staining was observed in neuronal projections of rat midbrain tissue sections.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Mouse Striatum were probed with Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Dopamine Transporter (~65 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or human Dopamine Transporter peptide (Run 2) or non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1525, Anti-Dopamine Transporter, clone 1D2 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Dopamine Transporter peptide displayed a KD value of 2.4 x 10-8 whereas its binding to non-specific control peptide displayed a KD of 1.5 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1D2, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 68.49 kDa |
| observed mol wt ~65 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat |
| species reactivity (predicted by homology) | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q01959  |
| Application: | Anti-Dopamine Transporter, clone 1D2 ZooMAb, Cat. No. ZRB1525, is a recombinant Rabbit monoclonal antibody that targets Dopamine Transporter (DAT) and is tested Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Dopamine Transporter in mouse brain and rat brain tissue sections. Affinity Binding Assay: A representative lot of this antibody bound human Dopamine Transporter peptide with a KD of 2.4 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Dopamine transporter. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1D2 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Dopamine transporter. It targets an epitope within 18 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Sodium-dependent dopamine transporter (UniProt: Q01959; also known as DA transporter, DAT, Solute carrier family 6 member 3) is encoded by the SLC6A3 (also known DAT1) gene (Gene ID: 6531) in human. Dopamine transporter is a multi-pass membrane protein with one extracellular domain (aa 161-237), two cytoplasmic domains (aa 1-68 and 589-620), and twelve transmembrane domains. It serves as an sodium/chloride-dependent amine transporter protein that pumps dopamine out of the synaptic cleft back into cytosol and terminates the effects of dopamine. It is shown to be highly expressed in substantia nigra. The dopamine transporter has been a target of several drugs, including psychostimulants, such as amphetamine and cocaine and is also subject to short- and long-term regulation by several drugs and mechanisms. Mutations in SCL6A3 gene are known to cause Infantile Parkinsonism-dystonia that is characterized by infantile onset of parkinsonism and dystonia along with global developmental delay and bradykinesia. Mice deficient in dopamine transporter exhibit reduced bone mass due to diminished cancellous bone volume, increased trabecular spacing, and reduced trabecular volume. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |