Anti-SEC61B Antibody, clone 1G14 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1511 - recombinant, expressed in HEK 293 cells
Synonym: Protein transport protein Sec61 subunit b
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 Dilution of Cat. No. ZRB1511, Anti-SEC61B, clone 1G14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. It positively stains the endoplasmic reticulum.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 Dilution of Cat. No. ZRB1511, Anti-SEC61B, clone 1G14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. It positively stains the endoplasmic reticulum.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Pancreas (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 Dilution of Cat. No. ZRB1511, Anti-SEC61B, clone 1G14 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. It positively stains the endoplasmic reticulum.
Western Blotting Enhanced Validation - Recombinant Antibody Technology HepG2 (Lane 1) and L6 (Lane 2) lysates were probed with Cat. No. ZRB1511, Anti-SEC61B, clone 1G14 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SEC61B (~10 kDa).
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 mg of recombinant SEC61B protein was analyzed by ELISA using various dilutions (starting at 3 mg/mL) of Cat. No. ZRB1511, Anti-SEC61B, clone 1G14 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1G14, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 9.97 kDa |
| observed mol wt ~10 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_006799  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human |
| species reactivity (predicted by homology) | canine, bovine, feline, mouse, zebrafish, monkey |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P60468 |
| Application: | Quality Control Testing Evaluated by Western Blotting in L6 cell lysate. Western Blotting Analysis: A 1,000 dilution of this antibody detected SEC61B in L6 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SEC61B in HepG2 cell lysate. ELISA Analysis: Various dilutions from a representative lot of this antibody detected SEC61B in ELISA application. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SEC61B in human pancreas tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Protein transport protein Sec61 subunit beta (SEC61B). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1G14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Protein transport protein Sec61 subunit b (SEC61B). It targets an epitope within 14 amino acids from the C-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Protein transport protein Sec61 subunit beta (UniProt: P60468; also known as Sec61B) is encoded by the SEC61B gene (Gene ID: 10952) in human. The transport of precursor proteins into and across the endoplasmic reticulum (ER) membrane is a highly conserved process. ER harbors the protein translocon that facilitates membrane integration or translocation of newly synthesized proteins targeted to multiple organelles of the endocytotic and exocytotic pathways. This translocon comprises the polypeptide-conducting SEC61 channel and several additional proteins and complexes that are permanently or transiently associated with the heterotrimeric SEC61 complex, which is composed of a, b, and g subunits. SEC61 is the central element of the protein translocation machinery and it forms a protein conducting channel with an aqueous central pore. Precursor polypeptides can either completely cross the ER membrane through the Sec61 channel or they can exit into the lipid phase of the membrane if they contain hydrophobic transmembrane domains. In addition to its role in protein translocation, SEC61 also serves as a Ca2+ channel that allows a passive efflux of Ca2+ ions from the ER lumen. Hence, SEC61 can counteract the active import of Ca2+ into the ER lumen through the sarcoplasmic/ER Ca2+ ATPase. SEC61B, a single-pass membrane protein, is a component of the SEC61 complex with one cytoplasmic (aa 2-70) and one transmembrane (aa 71-91) domain. Loss-of-function SEC61B are known to cause a precursor-specific ER protein import defect and is linked to polycystic liver disease. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lang, S., et al. (2017). Front. Physiol. 8; 887; Linxweiler, M., et al. (2017). Signal Transduct. Target Ther. 2; Article 17002). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |