Anti-phospho-TrkB (Tyr816) Antibody, clone 2C21 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1495 - recombinant, expressed in HEK 293 cells
Synonym: BDNF/NT-3 growth factors receptor; EC:2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; Trk-B; TrkB tyrosine kinase; Tropomyosin-related kinase
Product Type: Chemical
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of rat hippocampal cells treated with BDNF was performed using a 1:100 dilution of Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of rat hippocampal cells treated with BDNF was performed using a 1:100 dilution of Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cell membrane.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of rat hippocampal cells treated with BDNF was performed using a 1:100 dilution of Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the Cell membrane.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from Untreated HEK293 cells (Lane 1) and HEK293 cells treated with BDNF (Lane 2; 50 ng/mL for 30 min) were probed with Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-TrkB (Tyr816) (~110 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of TrkB peptide phosphorylated on tyrosine 816 (Run 1) or non-phosphorylated control TrkB peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal. The binding of this antibody to TrkB peptide phosphorylated on tyrosine 816 displayed a KD value of 3.8 x 109 whereas its binding to non-phosphorylated control TrkB peptide displayed a KD of 1.4 x 103.
Peptide Inhibition Assay Enhanced Validation-Recombinant Antibody Technology Lysates from HEK293 cells treated with BDNF were probed with Cat. No. ZRB1495, Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Lane 1: Anti-phospho-TRKB (Tyr816), clone 2C21 tested on lysates from HEK293 cells treated with BDNF. Lane 2: Anti-phospho-TRKB (Tyr816), clone 2C21 treated with lysate from HEK293 cells treated with BDNF and phospho-Tyr816 peptide. Lane 3: Anti-phospho-TRKB (Tyr816), clone 2C21 tested on lysate from HEK293 cells treated with a control peptide. Arrow indicates phospho-TrkB (Tyr816) (~110 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 2C21, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 92 kDa |
| observed mol wt 110 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human |
| species reactivity (predicted by homology) | bovine, sheep, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| inhibition assay: suitable (peptide) | |
| western blot: suitable | |
| UniProt accession no. | P11686  |
| Application: | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-TrkB (Tyr816) in SH-SY5Y cells and in rat hippocampal cells treated with BDNF. Peptide Inhibition Assay: Target band detection in lysate from HEK293 cells treated with BDNF (50 ng/mL, 30 min) was prevented by preblocking of a representative lot TrkB peptide phosphorylated on tyrosine 816, but not the corresponding non-phosphopeptide. Affinity Binding Assay: A representative lot of this antibody bound TrkB peptide phosphorylated on tyrosine 816 with a KD of 3.8 x 10-9 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | This Anti-phospho-TrkB (Tyr816), clone 2C21 ZooMAb, Cat. No. ZRB1495, is a recombinant Rabbit monoclonal antibody that targets phospho-TrkB (Tyr816) and is tested in Affinity Binding Assay, Immunocytochemistry, Peptide Inhibition, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 14 amino acids surrounding phosphotyrosine 816 from the C-terminal region of human TrkB. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2C21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects TrkB phosphorylated on tyrosine 816. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Pulmonary surfactant-associated protein C (UniProt: P11686; also known as SP-C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5) is encoded by the SFTPC (also known as SFTP2) gene (Gene ID: 6440) in human. Pulmonary surfactant is a lipid-protein complex that promotes alveolar stability by lowering the surface tension at the air-fluid interface in the peripheral air spaces. Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. Four different surfactant-associated proteins have been described: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and two small hydrophobic proteins (SP-B and SP-C). SFTPC is synthesized with two pro-peptides (aa 1-23 and 59-197) that subsequently produce the SP-5 protein (aa 24-58). Mutations in SFTPC have been found in sporadic and familial idiopathic pulmonary fibrosis (IPF) in murine models. In human, mutations in SFTPC gene have been linked to Respiratory distress syndrome (RDS) in premature infants. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |