Anti-DNA Polymerase beta Antibody, clone 3F21 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1491 - recombinant, expressed in HEK 293 cells
Synonym: DNAP
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using 1:100 dilution of Cat. No. ZRB1491, Anti-DNA Polymerase β, clone 3F21 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was primarily observed in granule cells, with a lesser frequency of staining in subsets of cells in molecular layer of human cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using 1:100 dilution of Cat. No. ZRB1491, Anti-DNA Polymerase β, clone 3F21 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was primarily observed in granule cells, with a lesser frequency of staining in subsets of cells in molecular layer of human cerebellum tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human cerebellum (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using 1:100 dilution of Cat. No. ZRB1491, Anti-DNA Polymerase β, clone 3F21 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was primarily observed in granule cells, with a lesser frequency of staining in subsets of cells in molecular layer of human cerebellum tissue sections.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Lysates from Jurkat cells (Lane 1), L6 cells (Lane 2), NIH3T3 cells (Lane 3), and HeLa cells (Lane 4) were probed with Cat. No. ZRB1491, Anti-DNA Polymerase β, clone 3F21 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates DNA Polymerase β (~38 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 μg/mL of DNA Polymerase β peptide (Run 1) or non-specific control peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1491, Anti-DNA Polymerase β, clone 3F21 ZooMAb® Rabbit Monoclonal. The binding of this antibody to DNA Polymerase β peptide displayed a KD value of 1.2 x 108 whereas its binding to non-specific control peptide displayed a KD of 4.6 x 103.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 3F21, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 38.18 kDa |
| observed mol wt 38 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, rat, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q06609  |
| Application: | Anti-DNA Polymerase β, clone 3F21 ZooMAb, Cat. No. ZRB1491, is a Rabbit monoclonal antibody that specifically targets DNA polymerase β and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DNA Polymerase β in L6, HeLa, and NIH3T3 cell lysates. Immunohistochemistry (Paraffin) Analysis: 1:100 from a representative lot detected DNA Polymerase β in human cerebellum tissue sections. Affinity Binding Assay: A representative lot of this antibody bound DNA polymerase β peptide with a KD of 1.2 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human DNA polymerase β. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3F21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects DNA polymerase β. It targets an epitope within 13 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | DNA repair protein RAD51 homolog 1 (UniProt: Q06609; also known as HsRAD51, hRAD51, RAD51 homolog A) is encoded by the RAD51 (also known as RAD51A, RECA) gene (Gene ID: 5888) in human. RAD51 plays an important role in DNA repair through homologous recombination. It is highly expressed in testis and thymus and is also found in small intestine, placenta, colon, pancreas and ovary. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. It is shown to catalyze the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. Phosphorylation of RAD51 at threonine 309 by checkpoint kinase 1 (CHEK1) enhances its association with chromatin at sites of DNA damage and promotes DNA repair. It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress and its stress-induced increases are observed in the mitochondria. Mutations in RAD51 gene are observed in cases of invasive ductal carcinoma and in Fanconi anemia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hilario, J., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(2);361-368). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |