Anti-Phospho-ACC1-(Ser79) Antibody, clone 1E11 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1481 - recombinant, expressed in HEK 293 cells
Synonym: Acetyl-CoA carboxylase 1;EC:6.4.1.2;ACC-
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysate from A431 cells overnight serum starved and treated with 0.5 mM Oligomycin was probed with Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Phospho-ACC1-(Ser79) (~250 kDa).
Western Blotting Enhanced Validation - Recombinant Antibody Technology Constructs containing GST-tagged non-phosphorylated ACC1 (Lane 1) and GST-tagged ACC1 phosphorylated on serine 79 (Lane 2) were probed with Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Phospho-ACC1-(Ser79) (~30 kDa). GST-tagged ACC1 constructs courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine.
Peptide Inhibition Assay Enhanced Validation - Recombinant Antibody Technology Constructs containing GST-tagged ACC1 phosphorylated on serine 79 were probed with Cat. No. ZRB1481, Anti-Phospho-ACC1-(Ser79), clone 1E11 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Phospho-ACC1-(Ser79) (~30 kDa). Lanes 1. GST-tagged ACC1 phosphorylated on serine 79 without any peptide treatment. 2: GST-tagged ACC1 phosphorylated on serine 79 treated with Phospho-ACC1 (Ser79) peptide. 3: GST-tagged ACC1 phosphorylated on serine 79 and treated with non-phosphorylated ACC1 peptide. GST-tagged ACC1 constructs courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1E11, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminus |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 265.19 kDa |
| observed mol wt ~250 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_071529.1  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| western blot: suitable | |
| UniProt accession no. | P11497 |
| Application: | Quality Control Testing Evaluated by Western Blotting in lysate from NIH3T3 cells overnight starved and treated Oligomycin. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Acetyl-CoA Carboxylase 1 (ACC1) phosphorylated on serine 79 in lysate from NIH3T3 cells overnight starved and treated with 0.5 μM Oligomycin for 30 min. Tested applications Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected a construct containing GST-tagged ACC1 phosphorylated on serine 79 (Lane 2) but did not react with non-phosphorylated construct (Lane 1). (Phospho-ACC1 construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine). Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Phospho-ACC1-(Ser79) in human breast cancer tissue sections. Peptide Inhibition Assay: Target band detection in a lysate from NIH3T3 cells treated with Oligomycin (0.5 mM) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 79 from the N-terminal region of rat Acetyl-CoA carboxylase 1 (ACC1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1E11 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Acetyl-CoA carboxylase 1 (ACC1) phosphorylated on serine 79. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Acetyl-CoA carboxylase 1 (UniProt: P11497; also known as EC:6.4.1.2, ACC1, ACC-alpha) is encoded by the Acaca (also known as Acac) gene (Gene ID: 60581) in rat. Acetyl-CoA carboxylase 1 (ACC1) is a multi-subunit, biotin-dependent cytosolic enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Two isoforms of ACC have been reported, alpha and beta that are encoded by two different genes. ACC1 is highly enriched in lipogenic tissues. Its expression is observed in brain, placenta, skeletal muscle, pancreas, and adipose tissue. It is not detected in the liver. Its levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephospho |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |