Anti-MRC1 Antibody, clone 1C9 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1440 - recombinant, expressed in HEK 293 cells
Synonym: C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; CD206; Human mannose receptor; MMR; Macrophage mannose receptor 1; Macrophage mannose receptor 1-like protein 1; hMR
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human lung (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in macrophages of human lung tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human lung (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in macrophages of human lung tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation-Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human lung (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in macrophages of human lung tissue sections.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of RAW 264.7 cell line was performed using a 1:100 dilution of Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of RAW 264.7 cell line was performed using a 1:100 dilution of Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation-Recombinant Antibody Technology Immunofluorescent analysis of RAW 264.7 cell line was performed using a 1:100 dilution of Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation-Recombinant Antibody Technology Human Lymph Node (Lane 1) and Human Lung (Lane 2) tissue lysates were probed with Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MRC1 (~180 kDa).
Affinity Binding Assay Enhanced Validation-Recombinant Antibody Technology 100 mg/mL of non-specific peptide (Run 2) or MRC1 peptide (Run 1) were analyzed by affinity binding assay using Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal. The binding of this antibody to MRC1 peptide displayed a KD value of 4.6 x 108 whereas its binding to non-specific peptide displayed a KD of 1.5 x 105.
Flow Cytometry Enhanced Validation-Recombinant Antibody Technology Staining of one million RAW 264.7 cells was performed using 0.01 μg of Cat. No. ZRB1440, Anti-MRC1, clone 1C9 ZooMAb® Rabbit Monoclonal (Yellow histogram) or the equivalent amount of a Rabbit IgG isotype control (Grey histogram), followed by a PE-conjugated Donkey Anti-Rabbit secondary antibody.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1C9, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 166.01 kDa |
| observed mol wt 180 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P22897  |
| Application: | Anti-MRC1, clone 1C9 ZooMAb, Cat. No. ZRB1440, is a recombinant Rabbit monoclonal antibody that specifically targets MRC1 and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MRC1 in Human lymph node tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MRC1 in human lung tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRC1 in RAW 264.7 cells.Flow Cytometry Analysis: 0.01 μg from a representative lot detected MRC1 in RAW 264.7 cells. Affinity Binding Assay: A representative lot of this antibody bound MRC1 peptide with a KD of 4.6 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human Macrophage mannose receptor 1 (MRC1). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1C9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Macrophage mannose receptor 1 (MRC1). It targets an epitope within 25 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Macrophage mannose receptor 1 (UniProt: P22897; also known as MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, hMR, Macrophage mannose receptor 1-like protein 1, CD206, MRC1) is encoded by the MRC1 (also known as CLEC13D, CLEC13DL, MRC1L1) gene (Gene ID: 4360) in human. MRC1 is a single-pass type I membrane protein that mediates the endocytosis of glycoproteins by macrophages. It also functions in phagocytosis and plays a role in immune homeostasis by scavenging unwanted mannoglycoproteins. It is expressed on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells. It is a C-type lectin with eight C-type lectin domains and can bind both sulfated and non-sulfated polysaccharide chains. MRC1 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains a large extracellular domain (aa 19-1389), a transmembrane domain (aa 1390-13410), and a cytoplasmic tail (aa 1411-1456). The extracellular region includes an N-terminal cysteine-rich domain that binds glycoproteins bearing sulfated sugars that terminate in 4-SO4GalNAc, a fibronectin II (FNII) domain, and eight carbohydrate recognition domains that bind sugars like mannose and fucose with high affinity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |