Anti-p-Myosin LC 2 (Ser19) Antibody, clone 2C10 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1432 - recombinant, expressed in HEK 293 cells
Synonym: Myosin regulatory light polypeptide 9
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Uterus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1432, Anti-p-Myosin LC 2 (Ser19), clone 2C10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells & stroma of Human Uterus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Uterus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1432, Anti-p-Myosin LC 2 (Ser19), clone 2C10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells & stroma of Human Uterus tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Human Uterus (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1432, Anti-p-Myosin LC 2 (Ser19), clone 2C10 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells & stroma of Human Uterus tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from HEK293 (Lane 1), C6 (Lane 2), and C2C12 (Lane 3) cells were probed with Cat. No. ZRB1432, Anti-p-Myosin LC 2 (Ser19), clone 2C10 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p-Myosin LC 2 (Ser19) (~19 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-phosphorylated myosin light chain 2 control peptide (Run 1) or phosphorylated myosin light chain 2 (Ser19) peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1432, Anti-p-Myosin LC 2 (Ser19), clone 2C10 ZooMAb® Rabbit Monoclonal. The binding of this antibody to phosphorylated myosin light chain 2 (Ser19) peptide displayed a KD of 6.0 x 10-6, whereas binding to non-phosphorylated myosin control peptide displayed a KD of 3.6 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2C10, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | Unknown |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 19.83 kDa |
| observed mol wt ~19 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_006088  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | bovine, feline, porcine, monkey, canine |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P24844 |
| Application: | Quality Control Testing Evaluated by Western Blotting in lysates from C2C12 cells. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected phospho-Myosin light chain 2 (Ser19) in lysate from C2C12 cells. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-Myosin LC 2 (Ser19) in C6 and HEK293 cell lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-Myosin LC 2 (Ser19) in human uterus tissue sections. Affinity Binding Assay: A representative lot of this antibody bound phospho-Myosin Light Chain 2 (Ser-19) with a KD of 6.0 x 10-6 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 19 from the N-terminal region of human Myosin Light Chain 2 (MLC2). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2C10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects myosin light chain 2 phosphorylated on serine 19. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Myosin regulatory light polypeptide 9 (UniProt: P24844; also known as 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2 smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1) is encoded by the MLY9 (also known as MLC2, MRLC1, MYRL2) gene (Gene ID: 10398) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). MLC-2C plays an important role in regulation of both smooth muscle and non-muscle cell contractile activity via its phosphorylation. It is phosphorylated at threonine 18 and serine 19 by myosin light chain kinase (MLCK) in a Ca2+/CaM-dependent manner. Rho kinase (ROCK) is also known to phosphorylates MLC-2C at serine 19. Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells. Phosphorylation is also required for myotube formation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kumar, CC., et al. (1989). Biochemistry. 28(9); 4027-4035). |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |