Anti-Macrosialin/CD68 Antibody, clone 2L16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1427 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1427, Anti-Macrosialin/CD68, clone 2L16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was primarily observed in germinal centers, with a lesser frequency of staining present in non-germinal centers of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1427, Anti-Macrosialin/CD68, clone 2L16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was primarily observed in germinal centers, with a lesser frequency of staining present in non-germinal centers of human tonsil tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1427, Anti-Macrosialin/CD68, clone 2L16 ZooMAb® Rabbit Monoclonal. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was primarily observed in germinal centers, with a lesser frequency of staining present in non-germinal centers of human tonsil tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology U937 cell lysate was probed with Cat. No. ZRB1427, Anti-Macrosialin/CD68, clone 2L16 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Macrosialin/CD68 (~33 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or CD68 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1427, Anti-Macrosialin/CD68, clone 2L16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to CD68 peptide displayed a KD of 2.6 x 10-8, whereas binding to the non-specific control peptide displayed a KD of 1.4 x 10-2.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 mg of Microsailin/CD68 peptide and control peptide were analyzed by ELISA using various dilutions (starting at 3 mg/mL) of Cat. No. ZRB1427, Anti-Macrosialin/CD68, clone 2L16 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 2L16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 37.41 kDa |
| observed mol wt ~33 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_001242  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P34810 |
| Application: | Quality Control Testing Evaluated by Western Blotting in U937 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Macrosialin/CD68 in U937 cell lysate. Tested applications Affinity Binding Assay: A representative lot of this antibody bound CD68 with a KD of 2.6 x 10-8 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis): A 1:1,000 dilution from a representative lot detected Macrosialin/CD68 in human tonsil tissue sections. ELISA Analysis: A representative lot detected Macrosialin/CD68 peptide. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-Macrosialin/CD68, clone 2L16 ZooMAb®, Cat. No. ZRB1427, is a recombinant Rabbit monoclonal antibody that specifically targets of Macrosialin/CD68 and is used in Affinity Binding Assay, ELISA, Immunohistochemistry (Paraffin), and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Macrosialin/CD68. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2L16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects human Macrosialin/CD68. It targets an epitope within 16 amino acids from the N-terminal, extracellular domain. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Macrosialin (UniProt: P34810; also known as Gp110, CD68) is encoded by the CD68 gene (Gene ID: 968) in human. CD68 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-319), a transmembrane domain (aa 320-345), and a short cytoplasmic tail (aa 345-354). It also contains a mucin-like domain (aa 23-140) and has two 30 amino acid long repeats (aa 70-99 and 100-129), proline-rich hinge (aa 140-152), and a LAMP-like domain. CD68 is highly expressed in blood monocytes and tissue macrophages. Its expression is also observed in lymphocytes, fibroblasts, and endothelial cells. It is also found in many tumor cells and allows them to attach to selectin on vascular endothelium, facilitating their dissemination to secondary sites. CD68 is mainly located in the endosomal/lysosomal compartment but can rapidly shuttle to the cell surface. In macrophages, it plays a role in their phagocytic activities, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, which allows for homing of macrophages to particular sites. It has a good predictive value as a prognostic marker of survival in cancer subjects. CD68 expression is shown to be upregulated in macrophages in response to inflammatory stimuli, such as exposure to oxLDL, lipopolysaccharide, or interferon-g. Hence, it can serve as a good histochemical/cytochemica |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |