Anti-Angiopoietin-2 Antibody, clone 4L18 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1414 - recombinant, expressed in HEK 293 cells
Synonym: ANG-2; ANG2
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in trophoblasts of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in trophoblasts of human placenta tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human placenta (A) and Negative control (B) were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in trophoblasts of human placenta tissue sections.
Immunofluorescence
Enhanced Validation - Recombinant Antibody Technology
Confocal fluorescent analysis of post-fixed, frozen human placenta (A) and Negative control (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Enhanced Validation - Recombinant Antibody Technology
Confocal fluorescent analysis of post-fixed, frozen human placenta (A) and Negative control (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence
Enhanced Validation - Recombinant Antibody Technology
Confocal fluorescent analysis of post-fixed, frozen human placenta (A) and Negative control (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Enhanced Validation - Recombinant Antibody Technology
Confocal fluorescent analysis of post-fixed, frozen human placenta (A) and Negative control (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence
Enhanced Validation - Recombinant Antibody Technology
Confocal fluorescent analysis of post-fixed, frozen human placenta (A) and Negative control (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Enhanced Validation - Recombinant Antibody Technology
Confocal fluorescent analysis of post-fixed, frozen human placenta (A) and Negative control (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology C6 cell lysate was probed with Cat. No. ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Angiopoietin-2 (~48 kDa).
Affintiy Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of Angiopoietin-2 peptide (Run 1) and a non-specific control peptide (Run 2) were analyzed by affinity binding assay using ZRB1414, Anti-Angiopoietin-2, clone 4L18 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Angiopoietin-2 peptide displayed a KD of 4.6 x 10-7 whereas its binding to non-specific peptide displayed a KD of 2.6 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 56.92 kDa |
| observed mol wt ~48 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, rat |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | O15123  |
| Application: | Anti-Angiopoietin-2, clone 4L18 ZooMAb, Cat. No. ZRB1414, is a recombinant Rabbit monoclonal antibody that targets Angiopoietin-2 and is tested in Affinity Binding, Immunohistochemistry, Immunofluorescence, Western Blotting. |
| Application: | Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Angiopoietin-2 in post-fixed, frozen human placenta tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Angiopoietin-2 in human placenta tissue sections. Affinity Binding Assay: Affinity Binding Assay: A representative lot of this antibody bound Angiopoietin-2 peptide with a KD of 4.6 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugate linear peptide corresponding to 23 amino acids from the N-terminal region of human Angiopoietin-2. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4L18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Angiopoietin-2. It targets an epitope within 23 amino acids from the N-terminal region. |
| Storage and Stability: | Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Angiopoietin-2 (UniProt: O15123; also known as ANG-2) is encoded by the ANGPT2 gene (Gene ID: 285) in human. Angiopoietins are a family of growth factors that include Angiopoietin 1 and 2 and the orthologs 3 (in mouse) and 4 (in human). ANG-2 is a secreted glycoprotein that is expressed predominantly by endothelial cells and some smooth muscle cells in a tightly regulated manner. High expression of ANG-2 has been observed in placenta. In quiescent mature vessels its expression is shown to be low; however, upon receipt of inflammatory signals its levels are significantly elevated. ANG-2 binds to TEK/TIE2 and competes for the Angiopoietin-1 (ANG-1) binding site and modulates ANG-1 signaling. It is also reported to induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANG-1. Both ANG-1 and ANG-2 have been implicated in tumor angiogenesis and ANG-2 is highly up-regulated in the vasculature of mouse and human neoplasms. It also acts as a chemoattractant and mediates the migration of a monocytes that express Tie2 receptor. ANG-2 contains a fibrinogen C-terminal domain (aa 275-495) that mediates its interaction with the TEK/TIE2 receptor. Three isoforms of ANG-2 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Scholz, A., et al. (2015). Ann. N.Y. Acad. Sci. 1347; 45-51; Felcht, M., et al. (2012). J. Clin. Invest. 122(6); 1991-2005). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |