Anti-CD25 (IL2-RA) Antibody, clone 1K6 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1412 - recombinant, expressed in HEK 293 cells
Synonym: IL-2 receptor subunit alpha; IL-2-RA; IL-2R su+C4bunit alpha; IL2-RA; Interleukin-2 receptor subunit alpha; TAC antigen; p55
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1412, Anti-CD25 (IL2-RA), clone 1K6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in subsets of cells in germinal center & non-germinal centers of human tonsil sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1412, Anti-CD25 (IL2-RA), clone 1K6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in subsets of cells in germinal center & non-germinal centers of human tonsil sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human tonsil (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1412, Anti-CD25 (IL2-RA), clone 1K6 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Membranous/ECM staining was observed in subsets of cells in germinal center & non-germinal centers of human tonsil sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Human lymph node (Lane 1) and mouse spleen (Lane 2) tissue lysates were probed with Cat. No. ZRB1412, Anti-CD25 (IL2-RA), clone 1K6 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates CD25 (IL2-RA) (~35 kDa).
Flow Cytometry Enhanced Validation - Recombinant Antibody Technology Staining of one million human PBMC was performed using 1 μg of Cat. No. ZRB1412, Anti-CD25 (IL2-RA), clone 1K6 ZooMAb® Rabbit Monoclonal and visualized with a Donkey Anti-Rabbit secondary antibody and 1 μg of Rabbit IgG isotype control. Histogram represents the gated R1 population shown in the dot plot.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 30.82 kDa |
| observed mol wt ~35 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P01589  |
| Application: | Anti-CD25 (IL2-RA), clone 1K6 ZooMAb, Cat. No. ZRB1412, is a recombinant Rabbit monoclonal antibody that targets CD25 (IL2-RA) and is tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD25 (IL2-RA) in human lymph node tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD25 (IL2-RA) in human tonsil tissue sections. Flow Cytometry Analysis: 1μg from a representative lot detected CD25 (IL2-RA) in one million human peripheral blood mononuclear cells (PBMC). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | GST/His-tagged recombinant fragment corresponding to 119 amino acids from the N-terminal, extracellular domain of human IL-2 receptor subunit alpha (CD25). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1K6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects IL-2 receptor subunit alpha (CD25). It targets an epitope within 119 amino acids from the N-terminal region. |
| Storage and Stability: | Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Interleukin-2 receptor subunit alpha (UniProt: P01589; also known as IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25) is encoded by the IL2RA gene (Gene ID: 3559) in human. IL-2-RA is a non-covalent dimer of an alpha (CD25), beta unit (CD122). IL-2-RA is involved in the regulation of immune tolerance and controls the activity of T cells. IL-2RA can exist in three different forms; a high affinity dimer, an intermediate affinity monomer of beta subunit, and a low affinity monomer of alpha subunit. The high and intermediate affinity forms can also associate with a gamma subunit (CD132). CD25 is rapidly up-regulated upon activation of T cells. Activated T cells, regulatory T cells (Tregs) and NK cells express high levels of CD25, and high affinity expression of IL-2-RA is largely limited to these cells. CD25 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 22-240), a transmembrane domain (aa 241-250), and a short cytoplasmic tail (aa 260-272). It also contains two Sushi domains (aa 22-84 and 123-186). Mutations in IL2RA gene have been linked to insulin-dependent diabetes mellitus and immunodeficiency with lymphoproliferation that leads to recurrent viral, fungal, and bacterial infections. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. Ref.: (Driesen, J., et al. (2008). Immunobiology 213(9-10); 849-858). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |