Anti-Neurofilament M Antibody, clone 1B23 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1411 - recombinant, expressed in HEK 293 cells
Synonym: 160 kDa neurofilament protein; NF-M; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament triplet M protein
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human brain (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1411, Anti-Neurofilament M, clone 1B23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed neurons & neuropil of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human brain (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1411, Anti-Neurofilament M, clone 1B23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed neurons & neuropil of human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human brain (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1411, Anti-Neurofilament M, clone 1B23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed neurons & neuropil of human cerebral cortex tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1411, Anti-Neurofilament M, clone 1B23 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the neurofilaments, cytoskeleton.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1411, Anti-Neurofilament M, clone 1B23 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the neurofilaments, cytoskeleton.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of SH-SY5Y cells was performed using a 1:100 dilution of Cat. No. ZRB1411, Anti-Neurofilament M, clone 1B23 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the neurofilaments, cytoskeleton.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Mouse (Lane 1) and Rat (Lane 2) brain tissue lysates were probed with Cat. No. ZRB1411, Anti-Neurofilament M, clone 1B23 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Neurofilament M (~145 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | recombinant monoclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 102.47 kDa |
| observed mol wt ~145 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, mouse, human |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P07197  |
| Application: | Anti-Neurofilament M, clone 1B23 ZooMAb, Cat. No. ZRB1411, is a recombinant Rabbit monoclonal antibody that targets Neurofilament M and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Neurofilament M in mouse brain tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Neurofilament M in human brain tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Neurofilament M in SH-SY5Y cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal region of human Neurofilament M. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 0.03 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1B23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Neurofilament M. It targets an epitope within 150 amino acids from the C-terminal region. |
| Storage and Stability: | Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Neurofilament medium polypeptide (UniProt: P07197; also known as NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein) is encoded by the NEFM (also known as NEF3, NFM) gene (Gene ID: 4741) in human. Neurofilaments are intermediate filaments that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. They are built from three intertwined protofibrils, which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neurofilaments contain a number of repeats of the tripeptide KSP (Lys-Ser-Pro) and NF-M is phosphorylated on a number of the serines in this motif by protein kinase A and C. Phosphorylation of NF-M results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Deletion of the phosphorylated tail domain of NF-M is reported to inhibit radial growth of axons and reduce their conduction velocities. However, mice expressing NF-M subunits that lack phosphorylation at KSP sites along the tail domain have normal axon calibers and conduction velocities, indicating that the NF-M tail domain, but not tail phosphorylation is crucial for axon radial growth. The level of phosphorylation is shown to be altered developmentally and coincides with a change in the neurofilament function. Mutations in the rod domain region of NF-M have been identified in early onset Parkinson s disease. Also, in the brains of Alzheimer s disease patients, a reciprocal relationship between O-GlcNAcylation and phosphorylation of N-FM has been reported where a decreased O-GlcNAcylation is accompanied with increased KSP phosphorylation of NF-M. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yuan, A., et al. (2012). J. Cell Sci. 125(14); 3257-3263). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |