Anti-BIM Antibody, clone 2O18, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1375 - recombinant, expressed in HEK 293 cells
Synonym: BIM; Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Strong cytoplasmic/membranous staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human lung cancer (A) and Negative control (B tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Rare cytoplasmic staining was observed in cells from human lung cancer sections tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human lung cancer (A) and Negative control (B tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Rare cytoplasmic staining was observed in cells from human lung cancer sections tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human lung cancer (A) and Negative control (B tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Rare cytoplasmic staining was observed in cells from human lung cancer sections tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria, cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria, cytoplasm.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria, cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from RAW264.7 cells (Lane 1) and mouse spleen tissue (Lane 2) were probed with Cat. No. ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates BIM (~22 kDa).
Affintiy Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of BIM peptide (Run 1) and a non-specific peptide (Run 2) were analyzed by affinity binding assay using ZRB1375, Anti-BIM, clone 2O18 ZooMAb® Rabbit Monoclonal. The binding of this antibody to BIM peptide displayed a KD value of 3.9 x 10-7 whereas its binding to non-specific peptide displayed a KD of 3.2 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 2O18, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 22.17 kDa |
| observed mol wt ~22 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | rat, human, mouse |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | O43521  |
| Application: | Anti-BIM, clone 2O18 ZooMAb, Cat. No. ZRB1375, a recombinant Rabbit monoclonal antibody that targets BIM and is tested in Affinity Binding, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected BIM in mouse spleen tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BIM in human breast cancer and human lung cancer tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BIM in RAW264.7 cells. Affinity Binding Assay: A representative lot of this antibody bound BIM peptide with a KD value of 3.9 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human BIM. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 2O18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bcl-2-like protein 11 (BIM) protein. It targets an epitope within 19 amino acids from the N-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Bcl-2-like protein 11 (UniProt: O43521; also known as Bcl2-L-11, Bcl2-interacting mediator of cell death, BIM) is encoded by the BCL2L11 (also known as BIM) gene (Gene ID: 10018) in human. BIM is a pro-apoptotic member of the Bcl-2 family that plays an important role in Bax/Bak-mediated cytochrome c release and apoptosis in response to various intrinsic stimuli. Like other members of the Bcl-2 family, BIM possesses a hydrophobic C-terminus that helps it to localize to intracytoplasmic membranes. In normal healthy cells BIM is localized to the microtubule-associated dynein motor complex in association with the LC8 dynein light chain; however, when subjected to apoptosis stimuli, it dissociates from LC8 and translocates to the mitochondria where it interacts with Bcl-2 to block its anti-apoptotic activity. Its N-terminal plays a role keeping it bound to the cytoskeleton and removal of that allows it to translocate to mitochondria. BIM contains a BH3 motif (aa 148-162) that is essential for its interaction with Bcl-2 proteins. BIM can undergo phosphorylation at serine 69 by MAPK1/MAPK3 that leads to its interaction with tripartite motif containing 2 (TRIM2), an E3 ubiquitin ligase, and polyubiquitination, followed by proteasomal degradation. BIM-deficient mice display higher numbers of B cells, CD4 and CD8 single-positive T cells, macrophages and granulocytes in their periphery. BIM is also reported to be essential for the deletion of auto-reactive B and T cells. Lymphocytes lacking BIM are refractory to several stimuli, including cytokine deprivation, deregulated calcium ion flux. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: O Connor, L., et al (1998). EMBO J. 17(2); 384-395; Gogada, R., et al. (2013). J. Biol. Chem. 288(1); 368-381). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |