Anti-NCOA1 Antibody, clone 5B9, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1371 - recombinant, expressed in HEK 293 cells
Synonym: Class E basic helix-loop-helix protein 74; EC:2.3.1.48; NCoA-1; Nuclear receptor coactivator 1; Protein Hin-2; RIP160; Renal carcinoma antigen NY-REN-52; SRC-1; Steroid receptor coactivator 1; bHLHe74
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U-2 OS cell line was performed using a 1:100 dilution of Cat. No. ZRB1371, Anti-NCOA1, clone 5B9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U-2 OS cell line was performed using a 1:100 dilution of Cat. No. ZRB1371, Anti-NCOA1, clone 5B9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U-2 OS cell line was performed using a 1:100 dilution of Cat. No. ZRB1371, Anti-NCOA1, clone 5B9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology HEK293 cell lysate was probed with Cat. No. ZRB1371, Anti-NCOA1, clone 5B9 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NCOA1 (~175 kDa).
Affintiy Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of human recombinant NCOA1 (Run 1) and a non-specific protein (GST; Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1371, Anti-NCOA1, clone 5B9 ZooMAb® Rabbit Monoclonal. The binding of this antibody to recombinant NCOA1 displayed a KD value of 6.9 x 10-7 whereas its binding to non-specific peptide displayed a KD of 3.9 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 5B9, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 156.76 kDa |
| observed mol wt ~175 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q15788  |
| Application: | Anti-NCOA1, clone 5B9 ZooMAb, Cat. No. ZRB1371, a recombinant Rabbit monoclonal antibody that targets Nuclear Receptor Coactivator 1 (NCOA1) and is tested in Affinity Binding Assay, Immunocytochemistry, and Western Blotting. |
| Application: | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NCOA1 in U-2 OS cells. Affinity Binding Assay: A representative lot of this antibody bound recombinant human NCOA1 protein with a KD of 6.9 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged recombinant fragment corresponding to 235 amino acids from the N-terminal half of human Nuclear receptor coactivator 1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 5B9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Nuclear receptor coactivator 1 (NCOA1). It targets an epitope within 235 amino acids from the N-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Nuclear receptor coactivator 1 (UniProt: Q15788; also known as EC:2.3.1.48, NCoA-1, Class E basic helix-loop-helix protein 74, bHLHe74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, SRC-1) is encoded by the NCOA1 (also known as BHLHE74, SRC1) gene (Gene ID: 8648) in human. NCOA1 I a member of the SRC/p160 nuclear receptor coactivator family that is widely expressed. It serves as a nuclear receptor coactivator that directly binds nuclear receptors and plays crucial roles in the regulation of gene expression mediated by a wide spectrum of steroid receptors such as androgen receptor, estrogen receptor alpha, and beta. It also displays histone acetyltransferase activity toward H3 and H4. NCOA1 contains seven LXXLL motifs of which motifs 3, 4 and 5 are essential for the association with nuclear receptors and motif 7 increases the affinity for steroid receptors in vitro. It is recruited to AF2 domains of ligand-activated nuclear receptors (NR) through LXXLL motifs located in a central NR interaction domain. It contains both a basic-helix-loop-helix (bHLH) domain (aa 23-80) and a periodicity/aryl hydrocarbon receptor nuclear translocator (ARNT)/single-minded (PAS) domain (aa 109-180). The bHLH-PAS domain houses a nuclear localization sequence and interacts with the Baf57 subunit of the SWI/SNF ATP-dependent chromatin remodeling complex. It also has a serine/threonine-rich region within the -terminal half (between amino acids 300 and 600) that allows for various posttranslational modifications. Dysregulation of NCOA1 has been linked to a variety of cancers and its elevated levels are reported to enhance breast cancer cell proliferation and metastasis. A chromosomal aberration involving NCOA1 has also been shown to be a cause for rhabdomyosarcoma. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, L., et al. (2018). Cancer Med. 7(10); 5205-5216; Rollins, DA., et al. (2015). Mol. Endocrinol 29(4); 502-517). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |