Anti-p-ATM (Ser1981) Antibody, clone 1E19, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1370 - recombinant, expressed in HEK 293 cells
Synonym: A-T mutated; Ataxia telangiectasia mutated; EC:2.7.11.1; Serine-protein kinase ATM
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) human breast cancer (A) and Negative control (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in human breast cancer tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cell line was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells treated with camptothecin (10 mM, 4 h) was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells treated with camptothecin (10 mM, 4 h) was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of HeLa cells treated with camptothecin (10 mM, 4 h) was performed using a 1:100 dilution of Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysate from UV treated HeLa cells was probed with Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p-ATM (Ser1981) (~370 kDa).
Western Blotting Enhanced Validation - Recombinant Antibody Technology GST-tagged recombinant fragment of non-phosphorylated ATM (Lane 1) or phosphorylated ATM (pSer1981) (Lane 2) were probed with Anti-p-ATM (Ser1981), clone 1E19 ZooMAb®, Cat. No. ZRB1370, Rabbit monoclonal antibody (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates GST-tagged ATM fragment (~30 kDa).
Affintiy Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of phosphorylated ATM (pSer1981) (Run 1) and non-phosphorylated ATM peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal. The binding of this antibody to phosphor-ATM displayed a KD value of 8.2 x 10-8 whereas to non-phosphorylated ATM peptide displayed KD of 7.3 x 10-3.
Peptide Inhibition Enhanced Validation - Recombinant Antibody Technology Lysates from UV treated HeLa cells were probed with Cat. No. ZRB1370, Anti-p-ATM (Ser1981), clone 1E19 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system. Lane 1: HeLa cells UV treated. Lane 2: HeLa cells UV treated and incubated with Ser1981 phosphorylated ATM peptide. Lane 3: HeLa cells UV treeated and incubated with non-phosphorylated ATM peptide. Arrow indicates p-ATM (Ser1981) (~370 kDa).
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Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1E19, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 350.69 kDa |
| observed mol wt ~370 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry: suitable | |
| inhibition assay: suitable (peptide) | |
| western blot: suitable | |
| UniProt accession no. | Q13315  |
| Application: | Anti-p-ATM (Ser1981), clone 1E19 ZooMAb, Cat. No. ZRB1370, a Rabbit monoclonal antibody that targets p-ATM (Ser1981) and is tested in Affinity Binding, Immunohistochemistry, Peptide Inhibition Assay, and Western Blotting. |
| Application: | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p-ATM (Ser1981) in HeLa cells treated with camptothecin (10 mM; 4 h) Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected p-ATM (Ser1981) in human breast cancer tissue sections. Peptide Inhibition Assay: Target band detection in lysate from UV treated HeLa cells was prevented by pre-blocking of a representative lot with the phosphor-ATM peptide, but not with the corresponding non-phosphopeptide. Analysis: A 1:1,000 dilution from a representative lot was used with HeLa cells treated with UV light for peptide block analysis. Affinity Binding Assay: A representative lot of this antibody bound human phosphorylated ATM (pSer1981) with a KD of 8.26 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 15 amino acids surrounding phosphoserine 1981 from the C-terminal half of human ATM. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1E19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human ATM phosphorylated on serine 1981. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Serine-protein kinase ATM (UniProt: Q13315; also known as EC:2.7.11.1, Ataxia telangiectasia mutated, A-T mutated, ATM) is encoded by the ATM gene (Gene ID: 472) in human. ATM is a member of the phosphatidyl inositol 3-kinase-like kinase (PIKK) family that is activated in response to double-strand breaks (DSBs) induced by ionizing radiation and radiomimetic drugs. It is usually found localized near the damaged regions within several minutes indicting its damage-sensing role. After their recruitment to sites of DNA damage, ATM phosphorylate several intracellular substrates, including Chk1 and Chk2 that in turn target other proteins to induce cell-cycle arrest and allow DNA repair to proceed. In normal cells ATM is present as inert dimers or multimers, which dissociate into highly active ATM monomers following any DNA damage. During activation, ATM undergoes autophosphorylation on Ser1981 and the activated ATM undergoes additional phosphorylations and acetylation reactions. Defects in ATM signaling are commonly seen in human cancer cells and they affect the sensitivity of tumors to chemo- and radiation therapies. Mutations in ATM gene are known to cause Ataxia telangiectasia that is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and a strong predisposition to cancers. Defects in ATM may contribute to T-cell acute lymphoblastic leukemia and T-prolymphocytic leukemia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shiloh, Y., and Ziv, Y. (2013). Nat. Rev. Mol. Cell Biol. 14(4); 197-210). |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |