Anti-Cardiac Troponin I Antibody, clone 1F23, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1355 - recombinant, expressed in HEK 293 cells
Synonym: Troponin I
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human heart (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in cardiac myocytes of human heart muscle tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human heart (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in cardiac myocytes of human heart muscle tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human heart (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in cardiac myocytes of human heart muscle tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat heart (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in cardiac myocytes of rat heart muscle tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat heart (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in cardiac myocytes of rat heart muscle tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and rat heart (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in cardiac myocytes of rat heart muscle tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human heart muscle (B) tissue sections was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human heart muscle (B) tissue sections was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human heart muscle (B) tissue sections was performed using a 1:1,000 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoskeleton.
Immunocytochemistry Analysis: Immunofluorescent analysis of AC16 cells was performed using a 1:100 dilution of Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from Human (Lane 1) and Mouse (Lane 2) heart tissue were probed with Cat. No. ZRB1355, Anti-Cardiac Troponin I, clone 1F23 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cardiac Troponin I (~26 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 1F23, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 24.0 kDa |
| observed mol wt ~26 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, rat, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P19429  |
| Application: | Anti-Cardiac Troponin I, clone 1F23 ZooMAb, Cat. No. ZRB1355, a Rabbit monoclonal antibody that targets Cardiac Troponin I and is tested in Immunocytochemisty, Immunofluorescence, Immunohistochemistry and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cardiac Troponin I in human heart tissue lysate. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cardiac Troponin I in AC16 cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin I in human heart and rat heart tissue sections. Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected Cardiac Troponin I in human heart muscle tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated liner peptide corresponding to 14 amino acids from the C-terminal region of human Cardiac Troponin I. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1F23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects cardiac troponin I. It targets an epitope within 14 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Troponin I, cardiac muscle (UniProt: P19429; also known as Cardiac troponin I) is encoded by the TNNI3 (also known as TNNC1) gene (Gene ID: 7137) in human. Troponin is a complex that regulates the contraction of skeletal muscle. It consists of 3 subunits known as C, T, and I, which are located periodically along the thin filament of the myofibrils. Troponins are found in skeletal and cardiac muscle, but not in smooth muscle. Troponin C (TnC) binds calcium ions, Troponin I is the inhibitory subunit of troponin that inhibits actomyosin ATPase and Troponin T binds to tropomyosin. It also confers calcium-sensitivity to actomyosin ATPase. Amino acids region 32-79 of Troponin I is involved in binding to TnC and region 129-149 is involved in binding to both TnC and actin. Cardiac muscle Troponin I has 31 amino acid residues at its N-terminal that are not present in the skeletal muscle form. Hence, cardiac specificity of this isoform is shown to offer accuracy in diagnosis in acute or chronic skeletal muscle injury. Mutation in TNNI3 gene are reported to result in multiple types of cardiomyopathies that can result in premature death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, H-C., and Fajer, PG (1998). Biochemistry 37(19); 6628-6635; Skeik, N., and Patel, DC (2007). Int. J. Angiol. 16(2); 53-58). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |