Anti-IL-33 Antibody, clone 5K9, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1350 - recombinant, expressed in HEK 293 cells
Synonym: IL-1F11; IL-33; Interleukin-1 family member 11; Interleukin-33; NF-HEV; Nuclear factor from high endothelial venules
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW 264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1350, Anti-IL-33, clone 5K9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW 264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1350, Anti-IL-33, clone 5K9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of RAW 264.7 cells was performed using a 1:100 dilution of Cat. No. ZRB1350, Anti-IL-33, clone 5K9 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human tonsil (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1350, Anti-IL-33, clone 5K9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in subsets of non-germinal center cells of human tonsil tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human tonsil (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1350, Anti-IL-33, clone 5K9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in subsets of non-germinal center cells of human tonsil tissue sections.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human tonsil (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1350, Anti-IL-33, clone 5K9 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in subsets of non-germinal center cells of human tonsil tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Lysates from Rat spleen (Lane 1), human spleen (Lane 2), Jurkat cells (Lane 3), EL4 cells (Lane 4), and RAW264.7 cells (Lane 5) lwere probed with Cat. No. ZRB1350, Anti-IL-33, clone 5K9 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IL-33 (~33 kDa).
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 5K9, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 30.76 kDa |
| observed mol wt ~33 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human, rat, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | O95760  |
| Application: | Anti-IL-33, clone 5K9 ZooMAb, Cat. No. ZRB1350, is a recombinant Rabbit monoclonal antibody that specifically targets IL-33 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), an Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IL-33 in rat spleen, human spleen, EL4, and RAW264.7 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IL-33 in human tonsil tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IL-33 in RAW 264.7 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | GST/His-tagged recombinant fragment corresponding to 146 amino acids from the C-terminal region of human IL-33. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 5K9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Interleukin-33. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Interleukin-33 (UniProt: O95760; also known as IL-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV) is encoded by the IL33 (also known as C9orf26, IL1F11, NFHEV) gene (Gene ID: 90865) in human. IL-33 is a tissue-derived nuclear cytokine of the IL-1 family that is highly expressed in endothelial cells, epithelial cells and fibroblast-like cells. It is normally released from damaged and necrotic barrier cells and serves as an alarmin to promote activation of the innate immune system and removal of pathogens. It is considered as a ubiquitous immune modulator. IL-33 is expressed at high levels in endothelial venules in tonsils, Peyer patches, and mesenteric lymph nodes. It exerts its cytokine activity by forming a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the co-receptor IL1RAP. This complex activates NF-kappa B and MAP kinase signaling in target cells. IL-33 is also reported to be involved in the maturation of Th2 cells and inducing the secretion of T-helper type 2-associated cytokines. Although full-length IL-33 can signal via IL1R1 receptor, proteolytic processing by cathepsin G and neutrophil elastase produces C-terminal peptides that are shown to be more active than the unprocessed full-length protein. IL-33 can also undergo proteolytic cleavage by caspase-3 and -7 that results in its activation. IL-33 has a homeodomain-like HTH domain (aa 1-65) that mediates its nuclear localization and heterochromatin association. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liew, FY., et al. (2016). Nat. Rev. Immunol. 16; 676-689; Carrol, C., and Girard, JP (2018). Immunol. Rev. 281(1); 154-168). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |