Anti-Gab1 Antibody, clone 4B20, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1348 - recombinant, expressed in HEK 293 cells
Synonym: GRB2-associated binder 1; GRB2-
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human small intestine (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1348, Anti-Gab1, clone 4B20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human small intestine tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human small intestine (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1348, Anti-Gab1, clone 4B20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human small intestine tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human small intestine (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:100 dilution of Cat. No. ZRB1348, Anti-Gab1, clone 4B20 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in glandular cells & lamina propria of human small intestine tissue sections.
Western Blotting Enhanced Validation - Recombinant Antibody Technology Bovine aorta tissue lysate was probed with Cat. No. ZRB1348, Anti-Gab1, clone 4B20 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Gab1 (~100 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of non-specific peptide (Run 1) or Gab1 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1348, Anti-Gab1, clone 4B20 ZooMAb® Rabbit Monoclonal. The binding of this antibody to Gab1 peptide displayed a KD value of 4.0 x 10-6 whereas its binding to non-specific peptide displayed a KD of 8.9 x 10-4.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 4B20, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 76.62 kDa |
| observed mol wt ~100 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | bovine, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q13480  |
| Application: | Anti-Gab1, clone 4B20 ZooMAb, Cat. No. ZRB1348, is a recombinant Rabbit monoclonal antibody that specifically targets GRB2-associated-binding protein 1 (Gab1) and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Gab1 in fetal bovine heart endothelial (FBHE) cell lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Gab1 in human small intestine tissue sections. Affinity Binding Assay: A representative lot of this antibody bound Gab1 peptide with a KD of 4.0 x 10-6 in affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | KLH-conjugated Linear Peptide corresponding to 30 amino acids from the C-terminal region of Human Gab1. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 4B20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects GRB2-associated-binding protein 1 (Gab1). It targets an epitope within 30 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | GRB2-associated-binding protein 1 (UniProt: Q13480; also known as GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1; Gab1) is encoded by the GAB1 gene (Gene ID: 2549) in human. Gab1 is a scaffolding protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. It recruits PI-3 kinase and other effector proteins in response to the activation of many receptor tyrosine kinases (RTKs). Gab1 can be phosphorylated on at least 16 sites by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta, hepatocyte growth factor/scatter factor receptor, and epidermal growth factor receptor. Phosphorylation of Gab1 on tyrosine 627 and tyrosine 659 is shown to be critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. Tyrosine phosphorylation of Gab1 mediates interaction with several proteins that contain SH2 domains. Gab1 plays important roles in embryonic development and cell migration and is shown to be a critical component in the interaction between focal adhesion kinase and Met in cancer cell invasion. Gab1 is also reported to be a major target in linoleic acid (LA)-induced enhancement of tumorigenesis. Mutations in GAB1 gene are known to cause autosomal recessive deafness that is characterized by severe to profound hearing loss resulting from damage to the neural receptors of the inner ear or the nerve pathways to the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mattoon, DR., et al. (2004). BMC Biol. 2; 24; Mouradian, M., et al. (2014). Prostaglandins Leukot. Essent. Fatty Acids. 90(4); 105-115). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |