Anti-SOX17 Antibody, clone 3F7, ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1345 - recombinant, expressed in HEK 293 cells
Synonym: SOX-17; Transcription factor SOX-17
Product Type: Chemical
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human fallopian tube (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in mucosal epithelium of human fallopian tube tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human fallopian tube (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in mucosal epithelium of human fallopian tube tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human fallopian tube (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in mucosal epithelium of human fallopian tube tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human cervical (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in endocervical cells of human cervical tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human cervical (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in endocervical cells of human cervical tissue sections.
Immunohistochemistry (Paraffin) Enhanced Validation - Recombinant Antibody Technology Formalin Fixed Paraffin Embedded (FFPE) Negative control (A) and human cervical (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal. Reactivity was detected using an Goat Anti-Rabbit IgG and HRP-DAB. Nuclear staining was observed in endocervical cells of human cervical tissue sections.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human fallopian tube (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human fallopian tube (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human fallopian tube (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human cervix (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human cervix (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunofluorescence Enhanced Validation - Recombinant Antibody Technology Confocal fluorescent analysis of Negative control (A) and human cervix (B) tissue sections were performed using a 1:100 dilution of Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal and visualized with a secondary antibody conjugated to Alexa Fluor® 488 (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology MDCK cell lysate was probed with Cat. No. ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SOX17 (~60 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 μg/mL of non-specific protein (GST; Run 1) and recombinant SOX17 protein (Run 2) were analyzed by affinity binding assay using Cat. No ZRB1345, Anti-SOX17, clone 3F7 ZooMAb® Rabbit Monoclonal. The binding of this antibody to recombinant SOX17 displayed a KD value of 5.0 x 10-8 whereas its binding to non-specific protein (GST) showed a KD of 3.5 x 10-4.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit (recombinant) |
| clone | 3F7, monoclonal |
| recombinant monoclonal | |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 44.12 kDa |
| observed mol wt ~60 kDa | |
| packaging | pkg of 25 μL |
| product line | ZooMAb® learn more |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | canine, human |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9H6I2  |
| Application: | Anti-SOX17, clone 3F7 ZooMAb, Cat. No. ZRB1345, is a recombinant Rabbit monoclonal antibody that specifically targets SOX17 and has been tested for use in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Application: | Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SOX17 in human fallopian tube and human cervical tissue sections. Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected SOX17 in human fallopian tube and human cervical tissue sections. Affinity Binding Assay: A representative lot of this antibody bound recombinant SOX17 with a KD of 5.0 x 10-8 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
| Immunogen: | His-tagged recombinant fragment corresponding to 149 amino acids from the C-terminal region of human SOX-17. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3F7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects SOX-17. It targets an epitope within the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Transcription factor SOX-17 (UniProt: Q9H6I2; also known as SOX-17) is encoded by the SOX17 gene (Gene ID: 64321) in human. SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It binds to the sequences 5′-AACAAT-3 or 5′-AACAAAG-3′ and modulates transcriptional regulation via WNT3A. It is reported to act upstream of the Notch system and downstream of the canonical Wnt system and provides a link between the canonical Wnt and Notch signaling. It is involved in the regulation of the formation of cells that go on to develop into endodermal tissues, such as pancreas and liver. Although detected in multiple tissues, it is preferentially expressed in esophagus, stomach and small intestine. SOX17 required for normal looping of the embryonic heart tube and in embryonic and postnatal vascular development. Inactivation of SOX17 in endothelial cells in mouse embryo is reported to cause a lack of arterial differentiation and vascular remodeling resulting in death in utero. Embryonic stem cells that are deficient in SOX17 fail to differentiate into extraembryonic cell types and maintain expression of pluripotency-associated transcription factors. However, forced expression of SOX17 results in down-regulation of embryonic stem cell associated gene expression and activates genes that are involved in differentiation toward an extraembryonic endoderm cell fate. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Corada, M., et al. (2013). Nat. Commun. 4, 2609; Niakan, KK et al. (2010). Gene Dev. 24(3): 312-326). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |