Anti-MCU Antibody, clone 3N3 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1338 - recombinant, expressed in HEK 293 cells
Synonym: Calcium uniporter protein mitochondrial;HsMCU;Coiled-
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cells was performed using a 1:100 dilution of Cat. No. ZRB1338, Anti-MCU, clone 3N3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cells was performed using a 1:100 dilution of Cat. No. ZRB1338, Anti-MCU, clone 3N3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of A549 cells was performed using a 1:100 dilution of Cat. No. ZRB1338, Anti-MCU, clone 3N3 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the mitochondria.
Western Blotting Enhanced Validation - Recombinant Antibody Technology A549 (Lane 1), PANC-1 (Lane 2) and NIH3T3 (Lane 3) cell lysates were probed with Cat. No. ZRB1338, Anti-MCU, clone 3N3 ZooMAb® Rabbit Monoclonal (1:1,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MCU (~41 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or MCU peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1338, Anti-MCU, clone 3N3 ZooMAb® Rabbit Monoclonal. The binding of this antibody to MCU peptide displayed a KD of 2.0 x 10-6, whereas binding to non-specific control peptide displayed a KD of 2.7 x 10-3.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 3N3, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | C-terminal |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 39.87 kDa |
| observed mol wt ~41 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_612366  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | bovine, canine, rat, feline, monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q8NE86 |
| Application: | Quality Control Testing Evaluated by Western Blotting in A549 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MCU in A549 cell lysate. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCU in PANC-1 and NIH3T3 cell lysates. Affinity Binding Assay: A representative lot of this antibody bound MCU with a KD of 2.0 x 10-6 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MCU in A549 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Calcium uniporter protein, mitochondrial (MCU). |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 3N3 is a Rabbit recombinant monoclonal antibody that specifically detects Calcium uniporter protein, mitochondrial (MCU). It targets an epitope within 17 amino acids from the C-terminal region. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Calcium uniporter protein, mitochondrial (UniProt: Q8NE86; also known as HsMCU, Coiled-coil domain-containing protein 109A, MCU) is encoded by the MCU (also known as C10orf42, CCDC109A) gene (Gene ID: 90550) in human. MCU is a multi-pass inner mitochondrial membrane calcium uniporter that mediates calcium uptake into mitochondria. It is synthesized with a transit peptide (aa 1-50), which is subsequently cleaved off to generate the mature MCU protein that has two mitochondrial matrix domains (aa 51-233 and 284-351), two transmembrane domains (aa 234-256 and 266-283) and one mitochondrial Intermembrane domain (aa 257-265). Its activity is regulated by the mitochondrial calcium uptake 1 (MICU1) and MICU2. MICU1 is a single pass membrane protein that helps to stabilize the entire mitochondrial calcium uniporter complex. It also limits the amount of calcium that enters the cell during low concentrations of calcium. Also, it functions alongside MCU to keep the accumulated calcium inside the mitochondrial matrix. MICU2 works with MICU1 and MCU to reduce the amount of calcium coming into the matrix. At low calcium levels MCU activity is down-regulated by MICU1 and MICU2 and at higher calcium levels MICU1 increases MCU activity. Synaptic activity is also reported to transcriptionally represses MCU gene, via a mechanism involving the nuclear Ca2+ and CaM kinase-mediated induction of Npas4, resulting in the inhibition of NMDA receptor-induced mitochondrial Ca2+ uptake and preventing excitotoxic death. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Marchi, S., and Pinton, P. (2014). J. Physiol. 592(5); 829-839; Qiu, J., et al. (2013). Nat. commun. 4; Article 2034; De Stefani, D., et al. (2011). Nature 476 (7360); 336-340). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |