Anti-SOX-11 Antibody, clone 1F16 ZooMAb® Rabbit Monoclonal
SIGMA/ZRB1337 - recombinant, expressed in HEK 293 cells
Product Type: Chemical
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NTERA2 cells was performed using a 1:100 dilution of Cat. No. ZRB1337, Anti-SOX-11, clone 1F16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NTERA2 cells was performed using a 1:100 dilution of Cat. No. ZRB1337, Anti-SOX-11, clone 1F16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of NTERA2 cells was performed using a 1:100 dilution of Cat. No. ZRB1337, Anti-SOX-11, clone 1F16 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488 (Green). Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Western Blotting Enhanced Validation - Recombinant Antibody Technology GST-tagged human recombinant SOX-11 was probed with Cat. No. ZRB1337, Anti-SOX-11, clone 1F16 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SOX-11 (~80 kDa).
Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 100 mg/mL of non-specific control peptide (Run 1) or SOX-11 peptide (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1337, Anti-SOX-11, clone 1F16 ZooMAb® Rabbit Monoclonal. The binding of this antibody to SOX11 peptide displayed a KD of 1 x 10-12, whereas binding to non-specific control peptide displayed a KD of 4.1 x 10-4.
ELISA Enhanced Validation - Recombinant Antibody Technology 0.1 mg of recombinant human SOX-11 protein and GST control protein were analyzed by ELISA using various dilutions (starting at 3 mg/mL) of Cat. No. Cat. No. ZRB1337, Anti-SOX-11, clone 1F16 ZooMAb® Rabbit Monoclonal. Alkaline phosphatase-conjugated Goat Anti-Rabbit secondary antibody was used for detection.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | 1F16, recombinant monoclonal |
| conjugate | unconjugated |
| description | recombinant, expressed in HEK 293 cells |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| epitope sequence | N-terminal half |
| form | lyophilized |
| greener alternative category | Aligned |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| isotype | IgG |
| mol wt | calculated mol wt 46.68 kDa |
| observed mol wt ~80 kDa | |
| packaging | antibody small pack of 25 μL |
| product line | ZooMAb® learn more |
| Protein ID accession no. | NP_003099  |
| purified by | using Protein A |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine, porcine, horse, monkey |
| storage temp. | 2-8°C |
| technique(s) | affinity binding assay: suitable |
| ELISA: suitable | |
| immunocytochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P35716 |
| Application: | Quality Control Testing Evaluated by Western Blotting with recombinant human SOX-11. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human SOX-11. Tested applications Affinity Binding Assay: A representative lot of this antibody bound SOX11 with a KD of 1 x 10-12 in an affinity binding assay. ELISA Analysis: A representative lot detected recombinant human SOX-11 protein in ELISA application. proteins. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SOX-11 in NTERA2 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Application: | Anti-SOX-11, clone 1F16 ZooMAb®, Cat. No. ZRB1337, is a recombinant Rabbit monoclonal antibody that specifically detects SOX-11 and is tested for use in Affinity Binding Assay, ELISA, Immunocytochemistry, and Western Blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information. |
| General description: | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. |
| Immunogen: | KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human SOX-11. |
| Legal Information: | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
| Reconstitution: | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Specificity: | Clone 1F16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects SOX-11. It targets an epitope within 15 amino acids from the N-terminal half. |
| Storage and Stability: | Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws. |
| Target description: | Transcription factor SOX-11 (UniProt: P35716; also known as SOX-11) is encoded by the SOX11 gene (Gene ID: 6664) in human. Sox-11 is a neural transcription factor that is involved in the embryonic neurogenesis and plays a role in tissue modeling during development, neuronal cell proliferation, and survival. Its expression is primarily observed in the brain and heart and low expression is detected in the kidney, pancreas, and muscle. Its transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Sox-11 serves as a transcription factor and acts as a transcriptional activator. It binds cooperatively with Brn2 or Oct6, which enhances its transcriptional activation. Sox-11 activity is controlled by epigenetics and transcriptional mechanisms and by post-translational modifications. Its three highly conserved phosphorylation sites, serine 30, 133, and 137, surrounding the DNA-binding high-mobility group (HMG box), are reported to modulate its transcriptional activity. These sites are unique to Sox-11. Serine 30 phosphorylation is reported to promote its nuclear over cytoplasmic localization. Phosphorylation of serine 133 by PKA modulates dendrite development of adult-born dentate granule neurons and fine tunes the activated of selected target genes. Higher expression of Sox-11 has been shown in Mantle cell lymphoma. Mutations in SOX11 gene are known to cause Coffin-Siris syndrome 9 that is characterized multiple malformations and intellectual disability. Mice deficient in Sox-11 exhibit defects in multiple organs including the lung, heart, and pancreas. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Balta, EA., et al. (2018). Sci. Rep. 8; 16196; Mozos, A., et al. (2009). Haematologica. 94(11); 1555-1562). |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |