Anti-PARD-3 Antibody, clone 1N18 ZooMAb^® Rabbit Monoclonal

SIGMA/ZRB1331 - recombinant, expressed in HEK 293 cells

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-ZRB1331-25UL	25 µL	No Price
45-ZRB1331-4X25UL	25 µL	No Price

Immunocytochemistry Enhanced Validation - Recombinant Antibody Technology Immunofluorescent analysis of U2OS cells was performed using a 1:100 dilution of Cat. No. ZRB1331, Anti-PARD-3, clone 1N18 ZooMAb® Rabbit Monoclonal and visualized with a Goat Anti-Rabbit secondary antibody conjugated to Alexa Fluor® 488. Actin filaments have been labeled with phalloidin (Red). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell junctions.

Western Blotting Enhanced Validation - Recombinant Antibody Technology U2OS cell lysate was probed with Cat. No. ZRB1331, Anti-PARD-3, clone 1N18 ZooMAb® Rabbit Monoclonal (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PARD-3 (~151 kDa).

Affinity Binding Assay Enhanced Validation - Recombinant Antibody Technology 10 mg/mL of non-specific GST protein (Run 1) or recombinant PARD-3 protein fragment (Run 2) were analyzed by affinity binding assay using Cat. No. ZRB1331, Anti-PARD-3, clone 1N18 ZooMAb® Rabbit Monoclonal. The binding of this antibody to PARD-3 protein fragment displayed a KD of 1.0 x 10-12 whereas binding to non-specific GST protein displayed a KD of 5.3 x 10-5.

12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.

12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.

12 Principles of Green Chemistry: Principle 6—Design for Energy Efficiency. This product was re-engineered to utilize less energy in the manufacturing process.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified antibody
antibody product type	primary antibodies
biological source	rabbit
clone	1N18, recombinant monoclonal
conjugate	unconjugated
description	recombinant, expressed in HEK 293 cells
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
epitope sequence	N-terminal half
form	lyophilized
greener alternative category	Aligned
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry .
isotype	IgG
mol wt	calculated mol wt 108.54 kDa
	observed mol wt ~115 kDa
packaging	antibody small pack of 25 μL
product line	ZooMAb^® learn more
Protein ID accession no.	NP_062565
Quality Level	200
recombinant	expressed in HEK 293 cells
shipped in	ambient
species reactivity	human
storage temp.	2-8°C
technique(s)	affinity binding assay: suitable
	immunocytochemistry: suitable
	western blot: suitable
UniProt accession no.	Q8TEW0

Application:	Quality Control Testing Evaluated by Western Blotting in U2OS cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PARD-3 in U2OS cell lysate. Tested applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected PARD-3 in HeLa cell lysate. Affinity Binding Assay: A representative lot of this antibody bound PARD-3 with a KD of 1.0 x 10-12 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PARD-3 in U2OS cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Application:	Anti-PARD-3, clone 1N18 ZooMAb^®, Cat. No. ZRB1331, is a recombinant Rabbit monoclonal antibody that specifically targets PARD-3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
General description:	ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here.
Immunogen:	His-tagged recombinant fragment corresponding to 288 amino acids from the N-terminal half of human Partitioning defective 3 homolog (PARD-3).
Legal Information:	ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution:	300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity:	lone 1N18 is a is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Partitioning defective 3 homolog (PARD-3). It targets and epitope within the N-terminal half.
Storage and Stability:	Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description:	Partitioning defective 3 homolog (UniProt: Q8TEW0; also known as PAR-3, PARD-3, Atypical PKC isotype-specific-interacting protein, ASIP, CTCL tumor antigen se2-5, PAR3-alpha) is encoded by the PARD3 (also known as PAR3, PAR3A) gene (Gene ID: 56288) in human. PARD-3 is an adapter protein that is involved in asymmetrical cell division and cell polarization processes and plays a central role in the formation of epithelial tight junctions. It is localized along the cell-cell contact region. PARD-3 is shown to inhibit atherosclerosis onset by blocking endothelial inflammation. It also colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II. In its N-terminal half, it contains three PDZ domains and a conserved oligomerization domain that is shown to be essential for proper subapical membrane localization. Its second PDZ domain (aa 461-546) mediates interaction with membranes containing phosphoinositol lipids. Together with PARD6, atypical protein kinase C (aPKC), and the Rho GTPase CDC42, it forms a complex that regulates cytoskeleton re-organization, post-Golgi vesicle trafficking, microtubule network polarization, and asymmetric cell division. PARD-3 can undergo phosphorylation at serine 827 by PRKCZ and PRKCI at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation and promote dissociation of the complex with PARD6. Mutations in PARD3 gene cause neural tube defects leading to congenital malformations of the central nervous system. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hikita, T., et al. (2018). EMBO reports. 19; e45253).

WGK Germany

WGK 1

Storage Temp.

2-8°C

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